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Invitrogen
PMEL Monoclonal Antibody (HMB-45)
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PMEL Antibody (MA5-16712) in ICC/IF
Immunofluorescence analysis of PMEL was performed using 70% confluent log phase SK-MEL-5 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PMEL Monoclonal Antibody (HMB-45) (Product # MA5-16712) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32... View More
产品信息
MA5-16712
应用
建议稀释比
已发表文章
产品规格
已发表种属
Human,
Mouse
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
HMB-45
抗原
Extract of pigmented melanoma metastases from lymph nodes.
偶联物
形式
Liquid
保存条件
4°C or -20°C if preferred
运输条件
Wet ice
RRID
产品详细信息
A suggested positive control for immunohistochemical applications is melanoma. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
靶标信息
Melanoma is a malignant tumor of melanocytes which are found predominantly in skin but also in the bowel and the eye (see uveal melanoma). It is one of the rarer types of skin cancer but causes the majority of skin cancer related deaths.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
