Invitrogen

PML Recombinant Rabbit Monoclonal Antibody (ARC0125)

查看其他17个PML抗体

抗体检测数据 (1)

PML Antibody in Western Blot (WB)

PML Antibody in Western Blot (WB)

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PML Antibody (MA5-35330) in WB

Western blot analysis of PML in extracts of various cell lines. Samples were incubated with PML Monoclonal antibody (Product # MA5-35330) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3 min.

PML Antibody in Western Blot (WB)

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PML Antibody in Western Blot (WB) — Western blot analysis of PML in extracts of various cell lines. Samples were incubated with PML Monoclonal antibody (Product # MA5-35330) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3 min.

产品信息

MA5-35330

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

酶联免疫吸附实验 (ELISA)

1 µg/mL

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

ARC0125

抗原

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PML (P29590)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2849232

产品详细信息

Immunogen sequence: MEPAPARSPR PQQDPARPQE PTMPPPETPS EGRQPSPSPS PTERAPASEE EFQFLRCQQC QAEAKCPKLL PCLHTLCSGC LEASGMQCPI CQAPWPLGAD

靶标信息

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: E3 SUMO-protein ligase PML; PML/RARA fusion; pml1; probable transcription factor PML; promyelocytic leukemia; Promyelocytic leukemia protein; promyelocytic leukemia, inducer of; Protein PML; RING finger protein 71; RING-type E3 SUMO transferase PML; TRIM19; tripartite motif protein TRIM19; Tripartite motif-containing protein 19

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基因别名: 1200009E24Rik; AI661194; MYL; PML; PP8675; RGD1562602; RNF71; TRIM19

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UniProt ID: (Human) P29590, (Mouse) Q60953

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Entrez Gene ID: (Human) 5371, (Mouse) 18854, (Rat) 315713

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DNA binding protein binding zinc ion binding ubiquitin protein ligase binding SUMO binding protein homodimerization activity SMAD binding transcription coactivator activity metal ion binding protein heterodimerization activity cobalt ion binding molecular_function

参与通路

response to hypoxia regulation of protein phosphorylation transcription, DNA-templated regulation of transcription, DNA-templated protein complex assembly activation of cysteine-type endopeptidase activity involved in apoptotic process DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest cell cycle arrest transforming growth factor beta receptor signaling pathway common-partner SMAD protein phosphorylation SMAD protein import into nucleus negative regulation of cell proliferation intrinsic apoptotic signaling pathway in response to oxidative stress response to UV response to gamma radiation regulation of calcium ion transport into cytosol fibroblast migration negative regulation of angiogenesis protein sumoylation myeloid cell differentiation regulation of cell adhesion negative regulation of cell growth PML body organization positive regulation of telomere maintenance endoplasmic reticulum calcium ion homeostasis circadian regulation of gene expression response to cytokine regulation of circadian rhythm intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator entrainment of circadian clock by photoperiod proteasome-mediated ubiquitin-dependent protein catabolic process innate immune response cell fate commitment regulation of MHC class I biosynthetic process negative regulation of transcription, DNA-templated positive regulation of fibroblast proliferation retinoic acid receptor signaling pathway negative regulation of interleukin-1 beta secretion maintenance of protein location in nucleus defense response to virus interferon-gamma-mediated signaling pathway branching involved in mammary gland duct morphogenesis intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to interleukin-4 cellular senescence extrinsic apoptotic signaling pathway regulation of signal transduction by p53 class mediator negative regulation of viral release from host cell negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process regulation of double-strand break repair positive regulation of extrinsic apoptotic signaling pathway positive regulation of defense response to virus by host immune system process protein targeting apoptotic process cell aging intrinsic apoptotic signaling pathway in response to DNA damage viral process positive regulation of histone deacetylation negative regulation of telomere maintenance via telomerase negative regulation of translation in response to oxidative stress negative regulation of mitotic cell cycle rhythmic process negative regulation of interleukin-1 secretion protein stabilization negative regulation of telomerase activity positive regulation of apoptotic process involved in mammary gland involution intrinsic apoptotic signaling pathway by p53 class mediator regulation of nucleic acid-templated transcription positive regulation of apoptotic signaling pathway biological_process