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POLB Antibody (MA5-47074) in ICC/IF
Immunofluorescent analysis of POLB in HeLa cells. Cells were fixed in ice-cold MeOH for 10 min. Green: DNA polymerase beta stained by DNA polymerase beta monoclonal antibody (Product # MA5-47074) diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1,000. Blue: Fluoroshield with DAPI Scale bar= 10 µm.
产品信息
MA5-47074
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat,
Zebrafish
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
HL1512
抗原
Recombinant protein encompassing a sequence within the center region of human DNA polymerase beta. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2938146
产品详细信息
Store as concentrated solution.
Centrifuge briefly prior to opening vial.
靶标信息
DNA polymerase beta comprises an amino-terminal 8- kDa domain and a carboxy-terminal 31- kDa domain. The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity while the C-terminal domain has a nucleotidyltransferase activity. Mammalian DNA polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells, but treatment of cells with the DNA-alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulfonate up-regulates beta-pol level. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 5'-deoxyribose-phosphate lyase; 5'-dRP lyase; AP lyase; DNA pol beta; DNA polymerase beta; DNA polymerase beta subunit; DNA-directed DNA polymerase beta; MGC125976; POL B; Pol beta; polymerase (DNA directed), beta; polymerase (DNA) beta; polymerase B; polymerase-beta
基因别名: A430088C08Rik; POLB; wu:fa97a05; zgc:109924
UniProt ID: (Human) P06746, (Rat) P06766, (Mouse) Q8K409
Entrez Gene ID: (Human) 5423, (Rat) 29240, (Mouse) 18970, (Zebrafish) 554204
damaged DNA binding
DNA-directed DNA polymerase activity
DNA-(apurinic or apyrimidinic site) lyase activity
protein binding
microtubule binding
lyase activity
enzyme binding
metal ion binding
DNA binding
transferase activity
nucleotidyltransferase activity
DNA polymerase activity
DNA-directed DNA polymerase
DNA-dependent DNA replication
DNA repair
base-excision repair
base-excision repair, base-free sugar-phosphate removal
base-excision repair, gap-filling
base-excision repair, DNA ligation
pyrimidine dimer repair
nucleotide-excision repair, DNA gap filling
inflammatory response
cellular response to DNA damage stimulus
salivary gland morphogenesis
aging
intrinsic apoptotic signaling pathway in response to DNA damage
response to gamma radiation
somatic hypermutation of immunoglobulin genes
response to ethanol
lymph node development
spleen development
homeostasis of number of cells
neuron apoptotic process
response to hyperoxia
immunoglobulin heavy chain V-D-J recombination
DNA biosynthetic process
DNA replication
apoptotic process
somatic diversification of immunoglobulins
double-strand break repair via nonhomologous end joining
