Invitrogen

PPAR alpha Polyclonal Antibody

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抗体检测数据 (2)

PPAR alpha Antibody in Western Blot (WB)

PPAR alpha Antibody in Western Blot (WB)

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PPAR alpha Antibody (42-4600) in WB

Western blot analysis of PPAR-alpha was performed by loading 20 µg of Mouse Liver (lane1), Jurkat (lane2), HeLa (lane3), NIH/3T3 (lane4) and PC-12 (lane5) lysate using Novex®NuPAGE®4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk for 1 hour at room temperature. PPAR-alpha was detected at ~58 kDa using PPAR-alpha ... View More

PPAR alpha Antibody in Western Blot (WB)

PPAR alpha Antibody in Western Blot (WB)

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PPAR alpha Antibody in Western Blot (WB) — Western blot analysis of PPAR-alpha was performed by loading 20 µg of Mouse Liver (lane1), Jurkat (lane2), HeLa (lane3), NIH/3T3 (lane4) and PC-12 (lane5) lysate using Novex®NuPAGE®4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk for 1 hour at room temperature. PPAR-alpha was detected at ~58 kDa using PPAR-alpha Rabbit Polyclonal Antibody (Product # 42-4600) at 1-3 µg/mL in 5% skim milk at 4°C overnight on a rocking platform. Goat Anti-Rabbit IgG - HRP Secondary Antibody (G21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

PPAR alpha Antibody in Western Blot (WB) — Western blot analysis of mouse liver homogenates using Zymed Rb anti-PPARalpha (Product # 42-4600).

产品信息

42-4600

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

specific to Human PPAR alpha

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533525

靶标信息

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPAR's). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPAR's are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR's can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with retinoid X receptor (RXR) alpha.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: MGC2237; MGC2452; Nuclear receptor subfamily 1 group C member 1; peroxisome proliferative activated receptor, alpha; peroxisome proliferator; peroxisome proliferator activated receptor; peroxisome proliferator-activated nuclear receptor alpha variant 3; Peroxisome proliferator-activated receptor alpha; PPAR-alpha

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基因别名: 4933429D07Rik; AW742785; hPPAR; NR1C1; PPAR; PPAR-alpha; PPARA; PPARalpha

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UniProt ID: (Human) Q07869, (Mouse) P23204

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Entrez Gene ID: (Human) 5465, (Mouse) 19013

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RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II repressing transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding transcription coactivator binding DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding transcription factor binding drug binding zinc ion binding lipid binding phosphatase binding protein domain specific binding ubiquitin conjugating enzyme binding protein complex binding sequence-specific DNA binding NFAT protein binding MDM2/MDM4 family protein binding receptor activity metal ion binding protein heterodimerization activity C4 zinc finger nuclear receptor DNA-binding transcription factor zinc finger transcription factor gene-specific transcriptional regulator

参与通路

negative regulation of transcription from RNA polymerase II promoter response to hypoxia transcription initiation from RNA polymerase II promoter lipid metabolic process fatty acid metabolic process heart development epidermis development negative regulation of macrophage derived foam cell differentiation negative regulation of receptor biosynthetic process negative regulation of cholesterol storage negative regulation of sequestering of triglyceride fatty acid transport intracellular receptor signaling pathway positive regulation of fatty acid beta-oxidation negative regulation of protein binding negative regulation of appetite response to insulin circadian regulation of gene expression behavioral response to nicotine wound healing lipoprotein metabolic process regulation of circadian rhythm steroid hormone mediated signaling pathway cellular lipid metabolic process positive regulation of gluconeogenesis negative regulation of blood pressure negative regulation of glycolytic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fatty acid oxidation negative regulation of inflammatory response enamel mineralization regulation of glycolytic process by positive regulation of transcription from RNA polymerase II promoter regulation of cellular ketone metabolic process by positive regulation of transcription from RNA polymerase II promoter regulation of lipid transport by positive regulation of transcription from RNA polymerase II promoter negative regulation of neuron death negative regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of leukocyte cell-cell adhesion negative regulation of transcription regulatory region DNA binding glucose metabolic process transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter regulation of gene expression regulation of fatty acid metabolic process rhythmic process