Invitrogen

PSA-NCAM Monoclonal Antibody (12E3), eBioscience™

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2个已发表图片

8 篇参考文献

说明书

实验方案

技术支持

说明书

实验方案

技术支持

PSA-NCAM Antibody in Immunocytochemistry (ICC/IF)

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PSA-NCAM Antibody (14-9118-82) in ICC/IF

Immunofluorescence analysis of PSA-NCAM was performed using 70% confluent log phase IMR-32 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PSA-NCAM Monoclonal Antibody (12E3), eBioscience (Product # 14-9118-82) at 20 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Pl... View More

PSA-NCAM Antibody in Immunohistochemistry (IHC)

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产品信息

14-9118-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

Assay-Dependent

免疫组化（冰冻） (IHC (F))

Assay-Dependent

免疫细胞化学 (ICC/IF)

20 µg/mL

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgM

分类

Monoclonal

类型

Antibody

克隆号

12E3

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2572932

产品详细信息

Description: The monoclonal antibody 12E3 specifically recognizes polysialylated neural cell adhesion molecule (PSA-NCAM) in human, mouse and rat. Polysialic acid is a long homopolymer of sialic acid that is negatively charged and is attached to neural cell adhesion molecule (CD56) and serves as a regulator of NCAM function. PSA attachment to NCAM is associated with inhibited adhesion of neural cells. PSA-NCAM expression is highly regulated and corresponds to specific neural developmental windows in which neural precursors are migrating and during the process of axonal sprouting, guidance, and targeting. PSA-NCAM expression is prevalent during development of the brain, but in the adult becomes restricted to regions undergoing self-renewal or exhibiting plasticity such as the olfactory bulb, suprachiasmatic nucleus, hippocampus, hypothalamus, and specific spinal cord nuclei. PSA-NCAM is re-expressed during tumorigenesis and is also expressed on cell lines isolated from neuroblastomas and pheochromocytomas.

Applications Reported: This 12E3 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.

Applications Tested: This 12E3 antibody has been tested by immunocytochemistry of formaldehyde-fixed PC-12 (rat) cells and can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Polysialic acid is a long homopolymer of sialic acid that is negatively charged and is attached to neural cell adhesion molecule (CD56) and serves as a regulator of NCAM function. PSA attachment to NCAM is associated with inhibited adhesion of neural cells. PSA-NCAM expression is highly regulated and corresponds to specific neural developmental windows in which neural precursors are migrating and during the process of axonal sprouting, guidance, and targeting. PSA-NCAM expression is prevalent during development of the brain, but in the adult becomes restricted to regions undergoing self-renewal or exhibiting plasticity such as the olfactory bulb, suprachiasmatic nucleus, hippocampus, hypothalamus, and specific spinal cord nuclei. PSA-NCAM is re-expressed during tumorigenesis and is also expressed on cell lines isolated from neuroblastomas and pheochromocytomas.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Experimental Treatment with Edaravone in a Mouse Model of Spinocerebellar Ataxia 1.

International journal of molecular sciences

Sucha M,Benediktova S,Tichanek F,Jedlicka J,Kapl S,Jelinkova D,Purkartova Z,Tuma J,Kuncova J,Cendelin J

14-9118-82 was used in Immunohistochemistry to explore the potential of edaravone to slow down SCA1 progression in a mouse knock-in SCA1 model.

Mon Jun 26 00:00:00 EDT 2023

Neuroblasts contribute to oligodendrocytes generation upon demyelination in the adult mouse brain.

iScience

El Waly B,Bertet C,Paris M,Falque M,Milpied P,Magalon K,Cayre M,Durbec P

14-9118-82 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to demonstrate that SVZ neuroblasts fate conversion proceeds through formation of a non-proliferating transient cellular state co-expressing markers of both neuronal and oligodendrocyte identities.

Fri Oct 21 00:00:00 EDT 2022

Peak density of immature nerve cells occurs with high-grade dysplasia in intraductal papillary mucinous neoplasms of the pancreas.

The Journal of pathology

Trinh VQ,Roland JT,Wong J,Revetta F,Patel K,Shi C,DelGiorno KE,Carter BD,Tan MCB

14-9118-82 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to investigate neoplasia in nerves in resected intraductal papillary mucinous neoplasms of the pancreas.

Thu Sep 01 00:00:00 EDT 2022

Neurogenesis of medium spiny neurons in the nucleus accumbens continues into adulthood and is enhanced by pathological pain.

Molecular psychiatry

García-González D,Dumitru I,Zuccotti A,Yen TY,Herranz-Pérez V,Tan LL,Neitz A,García-Verdugo JM,Kuner R,Alfonso J,Monyer H

14-9118-82 was used in Immunohistochemistry to find that the number of newborn neurons in the NAc is dynamically regulated by persistent pain, suggesting that adult neurogenesis of MSNs is an experience-modulated process.

Wed Sep 01 00:00:00 EDT 2021

Arginase Inhibition Supports Survival and Differentiation of Neuronal Precursors in Adult Alzheimer's Disease Mice.

International journal of molecular sciences

Polis B,Srikanth KD,Gurevich V,Bloch N,Gil-Henn H,Samson AO

Published figure using PSA-NCAM monoclonal antibody (Product # 14-9118-82) in Immunohistochemistry

Sat Feb 08 00:00:00 EST 2020

Bisecting GlcNAc Is a General Suppressor of Terminal Modification of N-glycan.

Molecular & cellular proteomics : MCP

Nakano M,Mishra SK,Tokoro Y,Sato K,Nakajima K,Yamaguchi Y,Taniguchi N,Kizuka Y

14-9118 was used in Immunohistochemistry-immunofluorescence to unveil a new regulation mechanism of protein N-glycosylation.

Tue Oct 01 00:00:00 EDT 2019

Oxidative Stress-Tolerant Stem Cells from Human Exfoliated Deciduous Teeth Decrease Hydrogen Peroxide-Induced Damage in Organotypic Brain Slice Cultures from Adult Mice.

International journal of molecular sciences

Xiao L,Saiki C,Okamura H

14-9118 was used in Immunohistochemistry to study the protective effects of oxidative stress tolerant stem cells harvested from human exfoliated deciduous teeth.

Mon Apr 15 00:00:00 EDT 2019

Human Dental Pulp Cells Differentiate toward Neuronal Cells and Promote Neuroregeneration in Adult Organotypic Hippocampal Slices In Vitro.

International journal of molecular sciences

Xiao L,Ide R,Saiki C,Kumazawa Y,Okamura H

Published figure using PSA-NCAM monoclonal antibody (Product # 14-9118-82) in Immunofluorescence

Fri Aug 11 00:00:00 EDT 2017

生物信息学

蛋白别名: antigen recognized by monoclonal antibody 5.1H11; CD56; N-CAM-1; Neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; Polysialylated NCAM; PSA NCAM

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基因别名: CD56; E-NCAM; MSK39; N-CAM; N-CAM-1; NCAM; NCAM-1; NCAM-C; NCAM1; NCAMC

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UniProt ID: (Human) P13591, (Mouse) P13595, (Rat) P13596

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Entrez Gene ID: (Human) 4684, (Mouse) 17967, (Rat) 24586

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功能

virus receptor activity Ras guanyl-nucleotide exchange factor activity protein binding heparin binding phosphatase binding LRR domain binding identical protein binding fibroblast growth factor receptor binding cytoskeletal protein binding

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