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PSD-95 Antibody (MA5-45142) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using HaCaT cells. Fixation involved Cold 100% methanol for 10 minutes at -20°C. Samples were incubated with PSD95 monoclonal antibody (Product # MA5-45142) at 1:100 for 1 hour at RT, followed by FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filamentous-like staining.
产品信息
MA5-45142
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
7E3
抗原
Recombinant rat PSD-95
偶联物
激发/发射光谱
482/675 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
47.35mM phosphate/2.15mM MES, pH 7.2, with 2mM EDTA, 0.17066µM Pentachlorophenol, 0.8mM ethanol
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2931596
产品详细信息
1 µg/mL was sufficient for detection of PSD-95 on 20 µg rat brain tissue extract by ECL immunoblot analysis using Goat Anti-Mouse IgG: HRP as the secondary.|Detects approximately 100kDa. An additional protein of >100kDa is also detected. Additional cross-reactive bands are detected at approximately 75kDa and 50kDa in rat and mouse samples.
靶标信息
Post Synaptic Density 95 kDa (PSD-95), also known as synapse associated protein 90 kDa (SAP90), is one of a family of membrane-associated proteins found in the postsynaptic density in forebrain neurons and certain presynaptic structures in the cerebellum. Like other members of the family, PSD-95 has three 90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain. PSD-95 is believed to participate in the clustering of certain proteins, including NMDA receptors, Shaker-type potassium channels at the synaptic membrane in central nervous system (CNS) neurons. There are two principal modes of interaction between PSD-95 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits), and some of these proteins may interact with PSD-95 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD-95 in vitro through a pseudo-homotypic PDZ-PDZ interaction.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: discs large homolog 4; Disks large homolog 4; FLJ97752; FLJ98574; post synaptic density; post-synaptic density protein 95; Postsynaptic density protein 95; PSD-95; PSD-95 alpha 2b; PSD-95 beta; SAP-90; Synapse-associated protein 90; synapse-associated protein SAP90; Tax interaction protein 15
基因别名: DLG4; Dlgh4; PSD-95; PSD95; SAP-90; SAP90; SAP90A
UniProt ID: (Human) P78352, (Rat) P31016, (Mouse) Q5NCV6
Entrez Gene ID: (Bovine) 100137840, (Human) 1742, (Rat) 29495, (Mouse) 13385
guanylate kinase activity
Ras guanyl-nucleotide exchange factor activity
protein binding
protein C-terminus binding
ligand-gated ion channel activity
kinase binding
protein phosphatase binding
PDZ domain binding
beta-1 adrenergic receptor binding
D1 dopamine receptor binding
P2Y1 nucleotide receptor binding
protein complex binding
acetylcholine receptor binding
ionotropic glutamate receptor binding
neuroligin family protein binding
scaffold protein binding
receptor binding
protein kinase binding
glutamate receptor binding
structural molecule activity
neurexin family protein binding
scaffold/adaptor protein
MAPK cascade
negative regulation of receptor internalization
protein complex assembly
signal transduction
positive regulation of cytosolic calcium ion concentration
chemical synaptic transmission
nervous system development
learning
synaptic vesicle maturation
ion transmembrane transport
social behavior
protein localization to synapse
locomotory exploration behavior
positive regulation of GTPase activity
establishment of protein localization
establishment or maintenance of epithelial cell apical/basal polarity
GMP metabolic process
GDP metabolic process
regulation of long-term neuronal synaptic plasticity
positive regulation of synaptic transmission
neuromuscular process controlling balance
dendritic spine morphogenesis
vocalization behavior
AMPA glutamate receptor clustering
receptor localization to synapse
regulation of N-methyl-D-aspartate selective glutamate receptor activity
positive regulation of excitatory postsynaptic potential
regulation of grooming behavior
neuronal ion channel clustering
nucleotide phosphorylation
locomotory behavior
response to cocaine
regulation of neuronal synaptic plasticity
