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PSMB10 Antibody (PA5-141029) in ICC/IF
Immunocytochemistry analysis of PSMB10 in SH-SY5Y cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 , permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Samples were incubated in PSMB10 Polyclonal antibody (Product # PA5-141029) using a dilution of 1:200 in 2% negative goat serum overnight at 4 followed by Goat Anti-Rabbit IgG H&L (iFluor™ 488) secondary antibody at a dilution of 1:1,000. PBS instead of the primary antibody was use... View More
产品信息
PA5-141029
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein within huaman PSMB10 aa 40-273.
偶联物
形式
Liquid
浓度
1.86 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4, with 40% glycerol, 0.1% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2932481
靶标信息
The 20S proteasome is a protease complex that is responsible for cytosolic protein degradation and generation of peptide ligands for major histocompatibility complex (MHC) class I molecules, either in their final form or in the form of amino-terminally extended precursors. Upon IFN-gamma stimulation of cells, three constitutively expressed subunits of the 20S proteasome are replaced by inducible subunits LMP2 (low-molecular mass polypeptide 2), LMP7, and MECL-1 (multicatalytic endopeptidase complex-like-1, LMP10). LMP2, LMP7, and MECL-1 subunits form immunoproteasomes, which are associated with more efficient class I antigen processing and presentation. Independent assortment of LMP-2, LMP-7, and MECL-1 into different proteasome complexes can lead to 36 unique proteasome subsets, which may mediate differences in the cleavage specificities/cleavage motifs of proteins subject to constitutive- and immuno-proteasomes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Low molecular mass protein 10; Macropain subunit MECl-1; MGC1665; Multicatalytic endopeptidase complex subunit MECl-1; OTTHUMP00000174858; prosome Mecl1; proteasome (prosome, macropain) subunit, beta type, 10; proteasome (prosomome, macropain) subunit, beta type 10; proteasome catalytic subunit 2i; Proteasome MECl 1; Proteasome MECl-1; proteasome subunit beta 7i; Proteasome subunit beta type-10; Proteasome subunit beta-2i; proteasome subunit MECL1; PSMB 10
基因别名: beta2i; LMP10; Mecl-1; MECL1; PSMB10
UniProt ID: (Human) P40306, (Mouse) O35955
Entrez Gene ID: (Human) 5699, (Mouse) 19171
MAPK cascade
protein polyubiquitination
cell morphogenesis
stimulatory C-type lectin receptor signaling pathway
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
regulation of cellular amino acid metabolic process
humoral immune response
viral process
anaphase-promoting complex-dependent catabolic process
tumor necrosis factor-mediated signaling pathway
NIK/NF-kappaB signaling
Fc-epsilon receptor signaling pathway
T cell proliferation
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of mRNA stability
T cell receptor signaling pathway
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
Wnt signaling pathway, planar cell polarity pathway
negative regulation of canonical Wnt signaling pathway
positive regulation of canonical Wnt signaling pathway
proteolysis
proteasomal ubiquitin-independent protein catabolic process
proteolysis involved in cellular protein catabolic process
