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Invitrogen
PTEN Monoclonal Antibody (2F4C9)
高级验证
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
图: 1 / 8
PTEN Antibody (32-5800) in ICC/IF
Immunofluorescent analysis of PTEN was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with PTEN Mouse Monoclonal Antibody (Product # 32-5800) at 0.5 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at r... View More
产品信息
32-5800
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Human
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
2F4C9
抗原
Recombinant protein derived from the PTEN protein.
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.1% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2533087
产品详细信息
32-5800 reacts specifically with the human PTEN protein. Reactivity was confirmed with cell lines which contain endogenous PTEN (NIH3T3, K652, and DU145 prostate cancer line) as well as transfected cell lines (Rat1 and 293T cells). This antibody detects the ~55 kDa PTEN protein and was successfully used in western blotting analysis of PTEN in breast cancer cell lines. No cross-reactivity to any other proteins has been detected.
靶标信息
PTEN was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The PTEN gene is a phosphatidylinositol-3, 4, 5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. PTEN negatively regulates intracellular levels of phosphatidylinositol-3, 4, 5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. PTEN plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Also, PTEN may be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form of PTEN induces less tumor suppressive ability.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Inositol polyphosphate 3-phosphatase; mitochondrial phosphatase and tensin protein alpha; mitochondrial PTENalpha; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog; phosphatase and tensin-like protein; phosphatidylinositol 3; Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; phosphatidylinositol-3; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; pten tumor suppressor
基因别名: 10q23del; 2310035O07Rik; A130070J02Rik; AI463227; B430203M17Rik; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN; PTEN1; TEP1
UniProt ID: (Human) P60484, (Mouse) Q542G1
Entrez Gene ID: (Human) 5728, (Mouse) 19211
magnesium ion binding
phosphatidylinositol-3-phosphatase activity
phosphoprotein phosphatase activity
protein serine/threonine phosphatase activity
protein tyrosine phosphatase activity
platelet-derived growth factor receptor binding
protein binding
protein tyrosine/serine/threonine phosphatase activity
lipid binding
anaphase-promoting complex binding
phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity
enzyme binding
protein kinase binding
PDZ domain binding
identical protein binding
inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity
phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity
hydrolase activity
phosphatase activity
protein phosphatase
regulation of cyclin-dependent protein serine/threonine kinase activity
angiogenesis
negative regulation of protein phosphorylation
regulation of B cell apoptotic process
protein dephosphorylation
phosphatidylinositol biosynthetic process
apoptotic process
neuron-neuron synaptic transmission
synapse assembly
central nervous system development
heart development
aging
response to nutrient
learning or memory
memory
locomotory behavior
cell proliferation
positive regulation of cell proliferation
negative regulation of cell proliferation
response to glucose
response to zinc ion
regulation of neuron projection development
negative regulation of phosphatidylinositol 3-kinase signaling
cell migration
dentate gyrus development
central nervous system neuron axonogenesis
negative regulation of cell migration
adult behavior
negative regulation of myelination
regulation of protein stability
negative regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle
regulation of synaptic transmission, GABAergic
central nervous system myelin maintenance
response to estradiol
regulation of cellular component size
regulation of myeloid cell apoptotic process
response to ATP
multicellular organismal response to stress
social behavior
peptidyl-tyrosine dephosphorylation
response to drug
maternal behavior
negative regulation of apoptotic process
protein kinase B signaling
endothelial cell migration
inositol phosphate metabolic process
response to ethanol
locomotor rhythm
negative regulation of cell size
negative regulation of organ growth
response to arsenic-containing substance
inositol phosphate dephosphorylation
phosphatidylinositol dephosphorylation
platelet-derived growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
regulation of axon regeneration
cardiac muscle tissue development
forebrain morphogenesis
brain morphogenesis
negative regulation of epithelial cell proliferation
negative regulation of phagocytosis
negative regulation of axonogenesis
protein stabilization
T cell receptor signaling pathway
positive regulation of sequence-specific DNA binding transcription factor activity
negative regulation of focal adhesion assembly
negative regulation of protein kinase B signaling
rhythmic synaptic transmission
canonical Wnt signaling pathway
synapse maturation
prepulse inhibition
male mating behavior
long-term synaptic potentiation
long term synaptic depression
prostate gland growth
dendritic spine morphogenesis
negative regulation of dendritic spine morphogenesis
negative regulation of ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
cellular response to hypoxia
negative regulation of ribosome biogenesis
negative regulation of cell aging
negative regulation of excitatory postsynaptic potential
presynaptic membrane assembly
postsynaptic density assembly
positive regulation of TRAIL-activated apoptotic signaling pathway
positive regulation of ubiquitin protein ligase activity
positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of G1/S transition of mitotic cell cycle
positive regulation of excitatory postsynaptic potential
negative regulation of synaptic vesicle clustering
lipid metabolic process
nervous system development
dephosphorylation
neuron projection development
cellular response to decreased oxygen levels
positive regulation of apoptotic process
regulation of cell cycle
positive regulation of apoptotic signaling pathway
