Invitrogen

PUM1 Polyclonal Antibody

PUM1 Antibody in Immunocytochemistry (ICC/IF)

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PUM1 Antibody (PA5-30327) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of PUM1 was performed in DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PUM1 Polyclonal Antibody (Product # PA5-30327) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

PUM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-30327

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

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产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 902 and 1186 of Human Pumilio 1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.49 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2547801

产品详细信息

Recommended positive controls: 293T, A431, Jurkat, Raji, mouse brain.

Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (93%), Xenopus laevis (97%), Chicken (98%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

PUM1 (pumilio homolog 1) is a sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. It is a member of the PUF family comprised of evolutionarily conserved RNA-binding proteins. PUM1 binds to an RNA consensus sequence, the Pumilio Response Element (PRE), and 5'-UGUANAUA-3' that is related to the Nanos Response Element (NRE). PUM1 mediates post-transcriptonal repression of transcripts via different mechanisms - acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Mutations to the gene can result in spinocerebellar ataxia 47.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

PUM1 Is Overexpressed in Colon Cancer Cells With Acquired Resistance to Cetuximab.

Frontiers in cell and developmental biology

Liu Q,Xin C,Chen Y,Yang J,Chen Y,Zhang W,Ye L

PA5-30327 was used in Western Blotting to suggest that PUM1 positively regulates DDX5 and acts as a promoter in cetuximab-resistant colon cancer cells.

Tue Apr 26 00:00:00 EDT 2022

PUM1 Is Overexpressed in Colon Cancer Cells With Acquired Resistance to Cetuximab.

Frontiers in cell and developmental biology

Liu Q,Xin C,Chen Y,Yang J,Chen Y,Zhang W,Ye L

PA5-30327 was used in Western Blotting to suggest that PUM1 positively regulates DDX5 and acts as a promoter in cetuximab-resistant colon cancer cells.

Tue Apr 26 00:00:00 EDT 2022

RNase L promotes the formation of unique ribonucleoprotein granules distinct from stress granules.

The Journal of biological chemistry

Burke JM,Lester ET,Tauber D,Parker R

PA5-30327 was used in Western Blot to demonstrate that RLBs represent a unique RNP granule with a protein and RNA composition distinct from that of SGs in response to dsRNA lipofection in human cells, using immunofluorescence, live-cell imaging, and MS-based analyses.