Invitrogen

PUMA delta/gamma Polyclonal Antibody

PUMA delta/gamma Antibody in Western Blot (WB)

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PUMA delta/gamma Antibody (PA5-116382) in WB

Western Blot analysis of PUMA using the PUMA Polyclonal Antibody (Product # PA5-116382) at a 1:2000 dilution. A549 whole cell lysates were loaded at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

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产品信息

PA5-116382

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 158-192 amino acids from human BBC3

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2901016

靶标信息

PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.

仅用于科研。不用于诊断过程。未经明确授权不得转售。