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PXR Antibody (PA5-102431) in ICC/IF
Immunocytochemistry analysis of PXR in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PXR polyclonal antibody (Product # PA5-102431) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-102431
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human NR1I2(Accession O75469), corresponding to amino acid residues L348-T398.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2851836
产品详细信息
Antibody detects endogenous levels of total NR1I2.
靶标信息
This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Nuclear receptor subfamily 1 group I member 2; nuclear receptor subfamily 1, group 1, member 2; nuclear receptor subfamily 1, group I, member 2; ONR 1; Orphan nuclear receptor PAR1; Orphan nuclear receptor PXR; PAR 1; PAR 2; PAR q; pregnane X nuclear receptor variant 2; Pregnane X receptor; pregnane X receptor (nuclear receptor sub family 1, group I, member 2); Steroid and xenobiotic receptor; SXR
基因别名: BXR; mPXR; NR1I2; ONR1; PAR; PAR1; PAR2; PARq; PRR; PXR; PXR.1; PXR.2; PXR1; SAR; SXR
UniProt ID: (Human) O75469, (Rat) Q9R1A7, (Mouse) O54915
Entrez Gene ID: (Human) 8856, (Rat) 84385, (Mouse) 18171
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
steroid hormone receptor activity
transcription coactivator activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
drug binding
zinc ion binding
DNA binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
metal ion binding
transcription, DNA-templated
xenobiotic metabolic process
positive regulation of gene expression
intracellular receptor signaling pathway
exogenous drug catabolic process
xenobiotic transport
steroid hormone mediated signaling pathway
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
drug export
transcription initiation from RNA polymerase II promoter
signal transduction
steroid metabolic process
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
