Invitrogen

PYCRL Monoclonal Antibody (OTI8H6)

PYCRL Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 11

PYCRL Antibody (MA5-25320) in ICC/IF

Immunofluorescent analysis of PYCRL in COS7 cells. Cells were transfected with a plasmid overexpressing PYCRL and probed with a PYCRL monoclonal antibody (Product # MA5-25320).

PYCRL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

产品信息

MA5-25320

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:150

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

1:100

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

OTI8H6

抗原

Full length human recombinant protein of PYCRL produced in HEK293T cell

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 1% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2725163

靶标信息

PYCRL encodes a protein that belongs to the pyrroline-5-carboxylate reductase family of enzymes. Members of this family catalyze the final step in proline biosynthesis, converting pyrroline-5-carboxylate to proline. Glutamate and ornithine are precursors in the synthesis of proline. The protein encoded by this gene is a cytoplasmic enzyme involved in the biosynthesis of proline from ornithine.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Loss of attachment promotes proline accumulation and excretion in cancer cells.

Science advances

Pilley SE,Hennequart M,Vandekeere A,Blagih J,Legrave NM,Fendt SM,Vousden KH,Labuschagne CF

MA5-25320 was used in Western Blotting to suggest that detachment induced accumulation and secretion of proline may contribute to tumor progression by supporting increased production of extracellular matrix and providing proline to surrounding stromal cells.