Invitrogen

Perilipin 1 Polyclonal Antibody

Perilipin 1 Antibody in Immunocytochemistry (ICC/IF)

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Perilipin 1 Antibody (PA1-1051) in ICC/IF

Immunofluorescence analysis of Perilipin A was performed using 70% confluent 3T3-L1 cells differentiated with StemPro Adipogenesis Supplement (Product # A10070-01) for 5 days. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Perilipin Rabbit Polyclonal Antibo... View More

Perilipin 1 Antibody in Western Blot (WB)

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产品信息

PA1-1051

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:4,000

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免疫组化（石蜡） (IHC (P))

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免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

2 µg/mL

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产品规格

种属反应

Mouse, Rat

已发表种属

Mouse, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to the residues C E(502) P I L G R T Q Y S Q L R K K S(517) of rat Perilipin A.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2167579

产品详细信息

PA1-1051 detects perilipin A from rat and 3T3-L1 cell extract.

PA1-1051 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~62 kDa protein representing perilipin A from 3T3-L1 cell extract

The PA1-1051 immunogen is a synthetic peptide corresponding to the residues C E(502) P I L G R T Q Y S Q L R K K S(517) of rat Perilipin A. This sequence is 93% conserved in humans. The PA1-1051 immunizing peptide (Cat. # PEP-169) is available for use in neutralization and control experiments.

靶标信息

Adipose tissue is an energy reserve in animals and is strictly regulated in nondomestic species. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), resistin, and perilipin. Perilipin is an intracellular neutral lipid droplet protein that is hormonally regulated. This protein is localized exclusively to the surface of lipid droplets. In response to lypotic stimuli, perilipin is phosphorylated by protein kinase A. Once activated, perilipin has inhibitory affects upon hormone-sensitive lipase (HSL), a protein that mediates the hydrolysis of triacylglycerol, the major form of stored energy in the body. Perilipin expression is limited to adipocytes and steroidogenic cells. There are currently two known isoforms, Perilipin A and B. Both of these proteins are encoded by a single-copy gene and are the result of differential splicing events.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Exosomal miR-1304-3p promotes breast cancer progression in African Americans by activating cancer-associated adipocytes.

Nature communications

Zhao D,Wu K,Sharma S,Xing F,Wu SY,Tyagi A,Deshpande R,Singh R,Wabitsch M,Mo YY,Watabe K

PA1-1051 was used in Immunocytochemistry, Western Blot, Immunohistochemistry to reveal a mechanism of the disparity in breast cancer progression and suggest a potential utility of miR-1304-3p and the associated SNP as biomarkers for predicting the outcome of African American patients.

Wed Dec 14 00:00:00 UTC 2022

RANKL from bone marrow adipose lineage cells promotes osteoclast formation and bone loss.

EMBO reports

Hu Y,Li X,Zhi X,Cong W,Huang B,Chen H,Wang Y,Li Y,Wang L,Fang C,Guo J,Liu Y,Cui J,Cao L,Weng W,Zhou Q,Wang S,Chen X,Su J

PA1-1051 was used in Immunohistochemistry (Paraffin), Immunocytochemistry to investigate cellular source of Receptor activator of NF-κB ligand for osteoclastogenesis.

Mon Jul 05 00:00:00 UTC 2021

RANKL from bone marrow adipose lineage cells promotes osteoclast formation and bone loss.

EMBO reports

Hu Y,Li X,Zhi X,Cong W,Huang B,Chen H,Wang Y,Li Y,Wang L,Fang C,Guo J,Liu Y,Cui J,Cao L,Weng W,Zhou Q,Wang S,Chen X,Su J

PA1-1051 was used in Immunohistochemistry (Paraffin), Immunocytochemistry to investigate cellular source of Receptor activator of NF-κB ligand for osteoclastogenesis.

Mon Jul 05 00:00:00 UTC 2021

Combined extracts of Moringa oleifera, Murraya koeingii leaves, and Curcuma longa rhizome increases energy expenditure and controls obesity in high-fat diet-fed rats.

Lipids in health and disease

Kundimi S,Kavungala KC,Sinha S,Tayi VNR,Kundurthi NR,Golakoti T,Davis B,Sengupta K

PA1-1051 was used in Western Blotting to show LI85008F reduces body fat mass and controls body weight gain in rats.

Fri Aug 28 00:00:00 UTC 2020

Adipogenic Differentiation of Thyroid Cancer Cells Through the Pax8-PPARγ Fusion Protein Is Regulated by Thyroid Transcription Factor 1 (TTF-1).

The Journal of biological chemistry

Xu B,O'Donnell M,O'Donnell J,Yu J,Zhang Y,Sartor MA,Koenig RJ

PA11051 was used in western blot to investigate the effects of pioglitazone in transgenic mice

Fri Sep 09 00:00:00 UTC 2016

Adipogenic Differentiation of Thyroid Cancer Cells Through the Pax8-PPARγ Fusion Protein Is Regulated by Thyroid Transcription Factor 1 (TTF-1).

The Journal of biological chemistry

Xu B,O'Donnell M,O'Donnell J,Yu J,Zhang Y,Sartor MA,Koenig RJ

PA11051 was used in western blot to investigate the effects of pioglitazone in transgenic mice

Fri Sep 09 00:00:00 UTC 2016

Genomic binding and regulation of gene expression by the thyroid carcinoma-associated PAX8-PPARG fusion protein.

Oncotarget

Zhang Y,Yu J,Lee C,Xu B,Sartor MA,Koenig RJ

PA1-1051 was used in western blot to characterize the thyroid carcinoma-associated PAX8-PPARG fusion protein and genomic binding and regulation of gene expression