Invitrogen

Phosphatidylinositol 4,5-bisphosphate Monoclonal Antibody (2C11)

Phosphatidylinositol 4,5-bisphosphate Antibody in Immunocytochemistry (ICC/IF)

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Phosphatidylinositol 4,5-bisphosphate Antibody (MA3-500) in ICC/IF

Immunofluoresence staining of PIP2 in HeLa cells using Product # MA3-500.

Phosphatidylinositol 4,5-bisphosphate Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phosphatidylinositol 4,5-bisphosphate Antibody in Immunohistochemistry (IHC)

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产品信息

MA3-500

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:200

免疫细胞化学 (ICC/IF)

1:200

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

其他PubMed (Misc)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Chemical

已发表种属

Chemical

宿主/亚型

Mouse / IgM

分类

Monoclonal

类型

Antibody

克隆号

2C11

抗原

Liposomes (prepared in PBS with lipid A, phosphatidylcholine (PC) and cholesterol) containing synthetic di-palmitoyl PtdIns(4,5)P2.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

ascites

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_568690

产品详细信息

MA3-500 detects PIP2 from human tissues.

MA3-500 has been successfully used in ELISA, Immunoprecipitation, Immunofluorescence, and Western blotting procedures.

The MA3-500 antigen is liposomes containing synthetic di-palmitoyl ptdlns(4,5)P2.

靶标信息

Phosphatidylinositol 4,5-bisphosphate or PIP2 is an important second messenger for a number of signaling processes. When certain extracellular signals are received, Phosphilipase C cleaves phosphatidylinositol into DAG and PIP2. PIP2 has been shown to interact with actin structural proteins including profiling, gelsolin, and cofilin, and demonstrates regulatory function of actin polymerization. It has also been shown to regulate vesicular transport by interacting with proteins like ADP-ribosylation factor and phospholipase D. PIP2 has also been shown to significantly affect intracellular calcium concentrations, which also regulate a variety of activities.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Bacterial protein MakA causes suppression of tumour cell proliferation via inhibition of PIP5K1α/Akt signalling.

Cell death & disease

Toh E,Baryalai P,Nadeem A,Aung KM,Chen S,Persson K,Persson JL,Uhlin BE,Wai SN

MA3-500 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to indicate that MakA could affect eukaryotic cell signalling pathways involved in lipid biosynthesis.

Tue Dec 06 00:00:00 EST 2022

Phosphoinositol-4,5-Bisphosphate Regulates Auditory Hair-Cell Mechanotransduction-Channel Pore Properties and Fast Adaptation.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Effertz T,Becker L,Peng AW,Ricci AJ

MA3-500 was used in Immunohistochemistry to study the role of phosphoinositol-4,5-bisphosphate in auditory mechanoelectrical transduction.

Wed Nov 29 00:00:00 EST 2017

Role of endoplasmic reticulum stress in acrolein-induced endothelial activation.

Toxicology and applied pharmacology

Haberzettl P,Vladykovskaya E,Srivastava S,Bhatnagar A

MA3-500 was used in Western Blot to suggest that exposure to acrolein induces ER stress and triggers the unfolded protein response and that NF-kappaB activation and stimulation of cytokine production by acrolein could be attributed, in part, to ER stress.

Thu Jan 01 00:00:00 EST 2009

Nuclear PtdIns(4,5)P2 assembles in a mitotically regulated particle involved in pre-mRNA splicing.

Journal of cell science

Osborne SL,Thomas CL,Gschmeissner S,Schiavo G

MA3-500 was used in immunocytochemistry, immunoprecipitation and western blot to characterize the components of the complex containing phosphatidylinositol(4,5)-bisphosphate

Sun Jul 01 00:00:00 EDT 2001

Generation of phosphatidylinositol-specific antibodies and their characterization.

Biochemical Society transactions

Thomas CL,Steel J,Prestwich GD,Schiavo G

MA3-500 was used in immunocytochemistry and western blot to produce and characterize anti-prolactin-induced protein-2 monoclonal antibodies

Sun Aug 01 00:00:00 EDT 1999

生物信息学

蛋白别名: C45H85O19P3; PIP2

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