Invitrogen

Phospho-AMPK alpha-1,2 (Thr172) Polyclonal Antibody

Phospho-AMPK alpha-1,2 (Thr172) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-AMPK alpha-1,2 (Thr172) Antibody (PA5-114550) in ICC/IF

Immunocytochemistry analysis of Phospho-AMPK alpha-1,2 (Thr172) in C2C12 cells. Samples were treated with PFA, permeabilized in 0.1% saponin prior to blocking in 10% serum (45 min at 37°C), and incubated with polyclonal antibody (Product # PA5-114550) at a dilution of 1:400 (1 hr at 37°C). Secondary staining was applied with Alexa Fluor 594 conjugated goat anti-rabbit IgG using a dilution of 1:600.

Phospho-AMPK alpha-1,2 (Thr172) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-AMPK alpha-1,2 (Thr172) Antibody in Western Blot (WB)

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产品信息

PA5-114550

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human PRKAA1(Accession Q13131), corresponding to amino acid residues around phosphorylated Thr172.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

sequential chromatography

保存液

PBS with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2899186

产品详细信息

Antibody detects endogenous levels of AMPK alpha 1 when phosphorylated at Thr183, or AMPK alpha 2 when phosphorylated at Thr172.

靶标信息

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; AAPK2; ACACA kinase; Acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMP-activated protein kinase alpha-2 variant B; AMP-activated protein kinase, alpha 1 catalytic subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK alpha 1; AMPK alpha 2; AMPK alpha-1 chain; AMPK alpha-2 chain; AMPK subunit alpha-1; AMPK subunit alpha-2; AMPK-alpha-1 chain; AMPK-alpha-2 chain; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; pAMPK; prkaa2; protein kinase, AMP-activated, alpha 1 catalytic subunit; protein kinase, AMP-activated, alpha 2 catalytic subunit; Tau-protein kinase PRKAA1

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基因别名: 2310008I11Rik; A830082D05; AI194361; AI450832; AL024255; AMPK; AMPK1; AMPK2; AMPKa1; AMPKa2; AMPKalpha1; AMPKalpha2; C130083N04Rik; PRKAA; PRKAA1; PRKAA2