Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 1
Phospho-BRD4 (Thr204) Antibody (PA5-117254) in WB
Western blot analysis of Phospho-BRD4 (Thr204) in extracts of NIH/3T3 cells. Samples were incubated with Phospho-BRD4 (Thr204) Polyclonal antibody (Product # PA5-117254) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. Lysates/protei... View More
产品信息
PA5-117254
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic phosphorylated peptide around T204 of human BRD4 (NP_4905971)
偶联物
形式
Liquid
浓度
0.97 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2901884
产品详细信息
Positive Samples: NIH/3T3
Immunogen sequence: ASTPP
靶标信息
BRD4, a ubiquitously expressed nuclear phosphoprotein, is a member of BET protein family. It is a human homolog of mouse MCAP and consists of 2 N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus. It is expressed as 2 isoforms; the longer variant has a C-terminus region with a praline and glutamine-rich domains. BRD4 is a chromatin-binding protein whose expression is induced in response to growth stimuli. BRD4 acts at different stages of the cell cycle by interacting with proteins including RFC and SPA-1 and regulates cell growth. It also has a role in regulating chromosomal dynamics during mitosis. BRD4 functions as a cellular target for E2 protein of papillomavirus.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: BRD4-NUT FUSION; bromodomain-containing 4; bromodomain-containing 5; Bromodomain-containing protein 4; chromosome-associated protein; fsh-like protein; MCAP; Mitotic chromosome-associated protein; Protein HUNK1
基因别名: BRD4; Brd5; CAP; HUNK1; HUNKI; MCAP; WI-11513
UniProt ID: (Human) O60885, (Mouse) Q9ESU6
Entrez Gene ID: (Human) 23476, (Rat) 362844, (Mouse) 57261
regulation of transcription involved in G1/S transition of mitotic cell cycle
transcription, DNA-templated
cellular response to DNA damage stimulus
positive regulation of G2/M transition of mitotic cell cycle
viral process
covalent chromatin modification
positive regulation of transcription elongation from RNA polymerase II promoter
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of transcription from RNA polymerase II promoter
regulation of inflammatory response
regulation of phosphorylation of RNA polymerase II C-terminal domain
inner cell mass cell proliferation
chromatin remodeling
protein phosphorylation
chromosome segregation
positive regulation of DNA binding
histone H4-K12 acetylation
histone H3-K14 acetylation
negative regulation of DNA damage checkpoint
regulation of transcription, DNA-templated
chromatin modification
