Invitrogen

Phospho-CD104 Integrin beta 4 (Tyr1494) Polyclonal Antibody

Phospho-CD104 Integrin beta 4 (Tyr1494) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-CD104 Integrin beta 4 (Tyr1494) Antibody (PA5-143711) in ICC/IF

Immunocytochemistry analysis of Phospho-CD104 Integrin beta 4 (Tyr1494) in control (Top) and pervanadate-treated A431 cells (Bottom). The cells were labeled with mouse monoclonal antibody anti-integrin beta4 (Cytoplasmic region) (left), Phospho-CD104 Integrin beta 4 (Tyr1494) polyclonal antibody (Product # PA5-143711) (middle), or Phospho-CD104 Integrin beta 4 (Tyr1526) polyclonal antibody (Product # PA5-14... View More

Phospho-CD104 Integrin beta 4 (Tyr1494) Antibody in Western Blot (WB)

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产品信息

PA5-143711

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Phospho-Integrin beta4 (Tyr-1494) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 1494 of human Integrin beta4. This peptide sequence is found in all three Integrin beta4 isoforms and is highly conserved in rat and mouse Integrin beta4.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

~0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA, 50% glycerol

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2942939

产品详细信息

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-Integrin beta4 (Tyr-1494) peptide (without carrier). The antibody detects a 200kDa* protein corresponding to the molecular mass of Integrin beta4 on SDS-PAGE immunoblots of A431 cells treated with pervanadate, but not in control cells. Similar results were also observed in src-transformed Hct116 cells treated with pervanadate.

靶标信息

Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。