Invitrogen

Phospho-CaMKII alpha/beta (Thr286) Polyclonal Antibody

Phospho-CaMKII alpha/beta (Thr286) Antibody in Western Blot (WB)

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Phospho-CaMKII alpha/beta (Thr286) Antibody (PA1-4614) in WB

Western blot of CaMKII alpha/beta in rat brain lysate showing specific immunolabeling of a band at ~50 kDa corresponding to the alpha-subunit and a ~60 kDa band corresponding to the beta-subunit of Phospho-CaMKII alpha/beta (Thr286) polyclonal antibody (Product # PA1-4614) in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot trea... View More

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产品信息

PA1-4614

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

1:1,000

产品规格

种属反应

Human, Mouse, Rabbit, Rat

已发表种属

Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286 of rat CAMK2A/B conjugated to KLH

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2259386

产品详细信息

This antibody is predicted to react with human, mouse and Xenopus based on 100% sequence homology.

This antibody is specific for the ~50 kDa alpha-CaM Kinase II subunit and the ~60 kDa beta-CaM Kinase II subunit phosphorylated at Thr286 in Western blots of rat brain lysates. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

靶标信息

Ca2+/Calmodulin-Dependent Protein Kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular response to a wide variety of intercellular signals (Kennedy, 1998; Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003; Soderling, 2000; Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003; Picciotto et al., 1993). Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduces LTP and learning (Elgersma et al., 2002).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Phosphorylation of the HCN channel auxiliary subunit TRIP8b is altered in an animal model of temporal lobe epilepsy and modulates channel function.

The Journal of biological chemistry

Foote KM,Lyman KA,Han Y,Michailidis IE,Heuermann RJ,Mandikian D,Trimmer JS,Swanson GT,Chetkovich DM

PA1-4614 was used in Western Blotting to highlight the potential of drugs targeting posttranslational modifications to restore TRIP8b phosphorylation to reduce excitability in TLE.

Fri Oct 25 00:00:00 EDT 2019

Time-dependent activation of MAPK/Erk1/2 and Akt/GSK3 cascades: modulation by agomelatine.

BMC neuroscience

Musazzi L,Seguini M,Mallei A,Treccani G,Pelizzari M,Tornese P,Racagni G,Tardito D

PA1-4614 was used in western blot to study modulation of agomelatine by time dependent activation of Akt/GSK3 and MAPK/Erk1/2 cascades

Tue Oct 21 00:00:00 EDT 2014

生物信息学

蛋白别名: alpha CaM kinase II; alpha-CaMKII; Ca++/calmodulin-dependent protein kinase II beta subunit; Ca2+/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase (CaM kinase) II beta; calcium/calmodulin-dependent protein kinase II alpha subunit; calcium/calmodulin-dependent protein kinase II alpha-B subunit; calcium/calmodulin-dependent protein kinase II beta; calcium/calmodulin-dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase type II beta chain; Calcium/calmodulin-dependent protein kinase type II subunit alpha; Calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II alpha subunit; CaM kinase II beta subunit; CaM kinase II subunit alpha; CaM kinase II subunit beta; CaM-kinase II alpha chain; CaM-kinase II beta chain; CaMK II; CaMK-II alpha subunit; caMK-II subunit alpha; caMK-II subunit beta; CaMKIINalpha; proline rich calmodulin-dependent protein kinase

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基因别名: CAM2; CAMK2; CAMK2A; CAMK2B; Camk2d; CAMKA; CAMKB; CaMKII; Ck2b; KIAA0968; mKIAA0968; PK2CDD; PKCCD; R74975