Invitrogen

Phospho-Caveolin 2 (Tyr19) Polyclonal Antibody, Alexa Fluor™ 488

Phospho-Caveolin 2 (Tyr19) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-Caveolin 2 (Tyr19) Antibody (PA1-060-A488) in ICC/IF

Immunofluorescent analysis of CAV-2 (green) in U2OS cells. The cells were fixed with 4% Paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a CAV-2 rabbit polyclonal antibody (Product # PA1-060-A488) at a concentration of 5 µg/mL in blocking buffer for 1 hour at room temperature. F-actin (re... View More

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产品信息

PA1-060-A488

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

5 µg/mL

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic phosphopeptide corresponding to residues M(14) A D D A (pY) S H H S G C(25) of mouse CAV2.

查看免疫原

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1.0mg/mL BSA

内含物

0.05% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Dry ice (international)

RRID

AB_2725017

产品详细信息

PA1-060 detects phospho-caveolin-2 Y19 from human and mouse samples. Immunofluorescent staining of phospho-caveolin-2 Y19 in NIH 3T3 cells using PA1-060-A488 results in a distinct punctate staining pattern at the cell periphery.

靶标信息

Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21-24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types.

仅用于科研。不用于诊断过程。未经明确授权不得转售。