Invitrogen

Phospho-Connexin 43 (Ser279) Polyclonal Antibody

Phospho-Connexin 43 (Ser279) Antibody in Western Blot (WB)

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Phospho-Connexin 43 (Ser279) Antibody (PA5-105955) in WB

Western blot analysis of Phospho-Connexin 43 (Ser279) in PMA treated K562 cell lysate (absence (-) or presence (+) of non-phospho and phospho peptide). Samples were incubated with Phospho-Connexin 43 (Ser279) polyclonal antibody (Product # PA5-105955).

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产品信息

PA5-105955

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human GJA1(Accession P17302), corresponding to amino acid residues around phosphorylated Ser279.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

sequential chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2817354

产品详细信息

Antibody detects endogenous levels of Connexin 43 / GJA1 only when phosphorylated at Ser279.

靶标信息

Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication. GFAP are membrane-spanning proteins that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is also targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. Mutations in the GFAP gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy, oculodentodigital dysplasia and heart malformations. Alternatively spliced transcript variants of GFAP have been found.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: alpha 1 connexin; connexin 43; Connexin-43; Cx-43; Cx43; CXA1; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; gap junction membrane channel protein alpha 1; gap junction protein, alpha 1, 43kDa; ODD; ODOD; SDTY3; Syndactyly type III

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基因别名: AU042049; AVSD3; AW546267; CMDR; Cnx43; connexin43; CX43; Cx43alpha1; Cxn-43; EKVP; Gja-1; GJA1; GJAL; HLHS1; HSS; Npm1; ODDD; PPKCA

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UniProt ID: (Human) P17302, (Mouse) P23242, (Rat) P08050

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Entrez Gene ID: (Human) 2697, (Mouse) 14609, (Rat) 24392

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