Invitrogen

Phospho-ERK1/2 (Thr202, Tyr204) Monoclonal Antibody (MILAN8R), eBioscience™

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

13个已发表图片

15 篇参考文献

查看其他63个ERK1/2抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

Phospho-ERK1/2 (Thr202, Tyr204) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Phospho-ERK1/2 (Thr202, Tyr204) Antibody (14-9109-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded human breast cancer tissue stained with 5 µg/mL of Anti-Human/Mouse phospho-ERK1/2 (T202/Y204) Purified followed by Anti-Mouse IgG Biotin, Avidin HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.

Phospho-ERK1/2 (Thr202, Tyr204) Antibody in Western Blot (WB)

Phospho-ERK1/2 (Thr202, Tyr204) Antibody in Flow Cytometry (Flow)

Phospho-ERK1/2 (Thr202, Tyr204) Antibody in Immunohistochemistry (IHC)

Phospho-ERK1/2 (Thr202, Tyr204) Antibody

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产品信息

14-9109-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

Assay-Dependent

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

MILAN8R

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2572926

产品详细信息

Description: This MILAN8R monoclonal antibody recognizes human and mouse extracellular signal-regulated kinases 1 and 2 (also known as ERK1/2, p44/p42, or MAPK3/1) when phosphorylated on T202/Y204. ERK1/2 belong to a family of conserved serine/threonine protein kinases known as mitogen-activated protein kinases (MAPKs) that are involved in many cellular programs such as proliferation, differentiation, motility, and survival. ERK1/2 signaling is activated in response to numerous extracellular stimuli including mitogens, growth factors, and cytokines. The primary activators of ERK1/2 are MEK1 and MEK2 which act by phosphorylating the activation loop residues T202/Y204 and T185/Y187 in ERK1 and ERK2, respectively. Several downstream targets of ERK1/2 have been identified, including p90RSK and the transcription factor Elk-1. ERK1/2 are negatively regulated by MAPK phosphatases, known as DUSPs or MKPs, as well as by chemical inhibitors of MEK including U0126 and PD98059. Disruption of the ERK pathway is common in many types of cancer.

Applications Reported: This MILAN8R antibody has been reported for use in intracellular staining followed by flow cytometric analysis, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunohistochemical staining, and immunocytochemistry. (Fluorochrome conjugated MILAN8R is recommended for use in intracellular flow cytometry.).

Applications Tested: This MILAN8R antibody has been tested by immunoblotting of stimulated Jurkat cells and can be used at less than or equal to 5 µg/mL. This MILAN8R antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and by immunocytochemistry of MeOH-fixed cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

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14-9109-82 was used in Western Blot to evaluate the anticancer and radio-sensitizing efficacy of Zinc Oxide-Caffeic Acid Nanoparticles (ZnO-CA NPs).

Mon Oct 11 00:00:00 EDT 2021

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14-9109-82 was used in Western Blot to evaluate the anticancer and radio-sensitizing efficacy of Zinc Oxide-Caffeic Acid Nanoparticles (ZnO-CA NPs).

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Scientific reports

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MM-129 as a Novel Inhibitor Targeting PI3K/AKT/mTOR and PD-L1 in Colorectal Cancer.

Cancers

Hermanowicz JM,Pawlak K,Sieklucka B,Czarnomysy R,Kwiatkowska I,Kazberuk A,Surazynski A,Mojzych M,Pawlak D

14-9109-82 was used in Western Blot to examine the pharmacodynamics of MM-129 as a novel promising drug candidate against colon cancer.

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Fibroblast subtypes define a metastatic matrisome in breast cancer.

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Published figure using Phospho-ERK1/2 (Thr202, Tyr204) monoclonal antibody (Product # 14-9109-82) in Immunohistochemistry

Thu Feb 27 00:00:00 EST 2020

Cancer cell-intrinsic expression of MHC II in lung cancer cell lines is actively restricted by MEK/ERK signaling and epigenetic mechanisms.

Journal for immunotherapy of cancer

Neuwelt AJ,Kimball AK,Johnson AM,Arnold BW,Bullock BL,Kaspar RE,Kleczko EK,Kwak JW,Wu MH,Heasley LE,Doebele RC,Li HY,Nemenoff RA,Clambey ET

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14-9109 was used in Immunohistochemistry to describe the features of a new, pathologically distinctive, acquired myopathy with an unusual pattern of scattered necrotic muscle fibers that are neighbored, surrounded, or invaded, by large, often multinucleated, histiocytic cells.

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Clinical and experimental immunology

Chu F,Hu Y,Zhou Y,Guo M,Lu J,Zheng W,Xu H,Zhao J,Xu L

Published figure using Phospho-ERK1/2 (Thr202, Tyr204) monoclonal antibody (Product # 14-9109-82) in Flow Cytometry

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Butyrate upregulates the TLR4 expression and the phosphorylation of MAPKs and NK-κB in colon cancer cell in vitro.

Oncology letters

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Published figure using Phospho-ERK1/2 (Thr202, Tyr204) monoclonal antibody (Product # 14-9109-82) in Western Blot

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Cas9-mediated excision of proximal DNaseI/H3K4me3 signatures confers robust silencing of microRNA and long non-coding RNA genes.

PloS one

Janga H,Aznaourova M,Boldt F,Damm K,Grünweller A,Schulte LN

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Journal of immunology (Baltimore, Md. : 1950)

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Heterogeneity of leukemia-initiating capacity of chronic myelogenous leukemia stem cells.

The Journal of clinical investigation

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Germline and somatic FGFR1 abnormalities in dysembryoplastic neuroepithelial tumors.

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生物信息学

蛋白别名: ERK-1; ERK-2; erk1 erk2; ERK1b; ERT1; ERT2; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; Microtubule-associated protein 2 kinase; mitogen activated protein kinase 1; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; MNK1; p42 MAP Kinase; p42-MAPK; p44 MAP kinase; p44-ERK1; p44-MAPK; pp42/MAP kinase; protein tyrosine kinase ERK2

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基因别名: 9030612K14Rik; AA407128; AU018647; C78273; ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; HS44KDAP; HUMKER1A; Mapk; MAPK1; MAPK2; MAPK3; Mnk1; Mtap2k; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3