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Phospho-GAB1 (Tyr659) Antibody (PA5-36806) in WB
Western blot analysis of Phospho-GAB1 (Tyr659) in Lane 1: HeLa cell lysate treated with insulin (100 nM,15mins), Lane 2: Raw264.7 cell lysate treated with insulin (100 nM,15mins), Lane 3: H9C2 cell lysate treated with insulin (100 nM,15mins). Samples were incubated with Phospho-GAB1 (Tyr659) polyclonal antibody (Product # PA5-36806) at a dilution of 1:500.
产品信息
PA5-36806
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic phosphopeptide derived from human Gab 1 around the phosphorylation site of Tyrosine 659
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2553745
产品详细信息
This antibody detects endogenous protein at a molecular weight of 76 and 110 kDa.
Purity is >95% by SDS-PAGE.
靶标信息
Gab1 is a 115 kDa multiple docking protein that plays an essential role in cellular growth, transformation and apoptosis. Gab1 can be phosphorylated by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), platelet-derived growth factor receptor beta] (PDGFRbeta]), hepatocyte growth factor/scatter factor receptor (HGFR/SFR or c-Met), and epidermal growth factor receptor (EGF), as well as in response to cell-cell adhesion. Gab1 is tyrosine phosphorylated on at least 16 sites, some of which serve as binding sites for phosphoatidylinositol 3-kinase (PI3K), Grb2, PLC gamma 1, Nck, and SHP2. Phosphorylation of Gab1 on tyrosines 627 and 659 is critical for its binding to SHP2, and for activation of the ERK/MAPK pathway in response to EGF.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: GRB2-associated binder 1; GRB2-associated binding protein 1; GRB2-associated binding protein 1 long isoform; GRB2-associated-binding protein 1; Growth factor receptor bound protein 2-associated protein 1; OTTHUMP00000220263
基因别名: AA408973; AW107238; GAB1
UniProt ID: (Human) Q13480, (Mouse) Q9QYY0
Entrez Gene ID: (Human) 2549, (Rat) 361388, (Mouse) 14388
response to oxidative stress
epidermal growth factor receptor signaling pathway
activation of JUN kinase activity
heart development
cell proliferation
insulin receptor signaling pathway
epidermis development
regulation of phosphatidylinositol 3-kinase signaling
regulation of cell migration
phosphatidylinositol-3-phosphate biosynthetic process
ERBB2 signaling pathway
phosphatidylinositol phosphorylation
platelet-derived growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
labyrinthine layer development
interleukin-6-mediated signaling pathway
activation of MAPK activity
signal transduction
positive regulation of MAPK cascade
