Invitrogen

Phospho-Histone H3.3 (Ser31) Recombinant Rabbit Monoclonal Antibody (JE62-92)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

查看其他12个Histone H3.3抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (5)
高级验证 (4)

Phospho-Histone H3.3 (Ser31) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-Histone H3.3 (Ser31) Antibody (MA5-44190) in ICC/IF

Immunofluorescence analysis of Histone H3.3 (phospho S31) was performed using 70% confluent log phase THP-1 cells treated with 100 ng/mL of nocodazole for 16 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Histone H3.3 (Ser31) Recombinant Rabb... View More

Phospho-Histone H3.3 (Ser31) Antibody in Western Blot (WB)

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产品信息

MA5-44190

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JE62-92

抗原

Synthetic phospho-peptide corresponding to residues surrounding ser31 of human histone h3.3

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS with 40% glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2926320

产品详细信息

Predicted band size: 15 kDa

Positive Control: Hela cell lysates

靶标信息

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

仅用于科研。不用于诊断过程。未经明确授权不得转售。