Invitrogen

Phospho-Histone H3 (Ser10) Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3个已发表图片

6 篇参考文献

查看其他142个Histone H3抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

Phospho-Histone H3 (Ser10) Antibody in Western Blot (WB)

图: 1 / 8

Phospho-Histone H3 (Ser10) Antibody (44-1190G) in WB

Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), HeLa treated with Trichostatin A (400nM for 16hr) (Lane 2), HeLa treated with Nocodazole (100 ng/mL for 18hr) (Lane 3), HeLa treated with Calyculin A (50 ng/mL for 30 min) (Lane 4), HL-60 (Lane 5) and HL-60 treated with Calyculin A (50 ng/mL for 30 min) (Lane 6). The blot was probed with Anti-Phosph... View More

Phospho-Histone H3 (Ser10) Antibody in Peptide array (Array)

Phospho-Histone H3 (Ser10) Antibody in Immunohistochemistry (IHC)

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产品信息

44-1190G

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

查看2篇已发表文章 2篇已发表文章

肽阵列 (Array)

1:1,000

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Phosphopeptide derived from the region of human Histone H3 that contains serine 10.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533589

靶标信息

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

SUN2 regulates mitotic duration in response to extracellular matrix rigidity.

Proceedings of the National Academy of Sciences of the United States of America

Belaadi N,Pernet L,Aureille J,Chadeuf G,Rio M,Vaillant N,Vitiello E,Lafanechère L,Loirand G,Guilluy C

44-1190G was used in Western Blot to examine how extracellular matrix (ECM) rigidity affects cell division.

Tue Nov 08 00:00:00 EST 2022

Girardia dorotocephala transcriptome sequence, assembly, and validation through characterization of piwi homologs and stem cell progeny markers.

Developmental biology

Almazan EMP,Lesko SL,Markey MP,Rouhana L

Published figure using Phospho-Histone H3 (Ser10) polyclonal antibody (Product # 44-1190G) in Immunohistochemistry

Mon Jan 15 00:00:00 EST 2018

Distinct Requirements for FGFR1 and FGFR2 in Primitive Endoderm Development and Exit from Pluripotency.

Developmental cell

Molotkov A,Mazot P,Brewer JR,Cinalli RM,Soriano P

44-1190G was used in Immunocytochemistry-immunoflourescence to study the distinct requirements for FGFR1 and FGFR2 in primitive endoderm development and exit from pluripotency.

Mon Jun 05 00:00:00 EDT 2017

Analysis of Stem Cell Motility In Vivo Based on Immunodetection of Planarian Neoblasts and Tracing of BrdU-Labeled Cells After Partial Irradiation.

Methods in molecular biology (Clifton, N.J.)

Tasaki J,Uchiyama-Tasaki C,Rouhana L

44-1190G was used in immunocytochemistry to utilize immunodetection of planarian neoblasts and tracing of BrdU-labeled cells after partial irradiation to analyze stem cell motility in vivo

Tue Aug 09 00:00:00 EDT 2016

Nuclear Calcium/Calmodulin-dependent Protein Kinase II Signaling Enhances Cardiac Progenitor Cell Survival and Cardiac Lineage Commitment.

The Journal of biological chemistry

Quijada P,Hariharan N,Cubillo JD,Bala KM,Emathinger JM,Wang BJ,Ormachea L,Bers DM,Sussman MA,Poizat C

44-1190G was used in western blot to determine role of CaMKIIdelta in cardiac progenitor cells

Fri Oct 16 00:00:00 EDT 2015

The SKP1-Cul1-F-box and leucine-rich repeat protein 4 (SCF-FbxL4) ubiquitin ligase regulates lysine demethylase 4A (KDM4A)/Jumonji domain-containing 2A (JMJD2A) protein.

The Journal of biological chemistry

Van Rechem C,Black JC,Abbas T,Allen A,Rinehart CA,Yuan GC,Dutta A,Whetstine JR

44-1190G was used in western blot to report that degradation of JMJD2A is regulated by the proteasome.

Fri Sep 02 00:00:00 EDT 2011

生物信息学

蛋白别名: H3 histone family, member A; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3/b; H3/d; H3/f; H3/j; H3S10ph; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 1, H3j; histone 2, H3a; histone 2, H3c; Histone 3; Histone Cluster 2 H3a; Histone H3.1; Histone H3.2; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o

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基因别名: H3; H3.2; H3/A; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3F1K; H3F2; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; HIST1H3A; HIST1H3B; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; HIST2H3C; HIST2H3D

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UniProt ID: (Human) P68431, (Human) Q71DI3