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Phospho-IR/IGF1R (Tyr1158) Antibody (44802A1) in ICC/IF
Immunofluorescence analysis of IR/IGF1R pY1158 Alexa Fluor® 488 conjugate (Product # 44802A1) was performed using 70% confluent log phase MCF-7 cells treated with 100 ng/mL of human insulin for 15 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Prim... View More
产品信息
44802A1
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic phosphopeptide derived from the region of IR/IGF1R that contains tyrosine 1158 of the human insulin receptor (IR) (tyrosine 1131 for IGF1R)
偶联物
激发/发射光谱
499/520 nm
查看光谱
形式
Liquid
纯化类型
Size-exclusion chromatography
保存液
Dulbecco's PBS, pH 7.3, with 0.2% BSA
内含物
0.05% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2802658
产品详细信息
This antibody is predicted to react with mouse and rat.
靶标信息
Insulin and Insulin-like Growth Factor receptors are transmembrane tyrosine kinase receptors that play critical roles in development, cell growth and metabolism. Insulin/Insulin-like growth factor-1 binding to the extracellular domain of IR and IGFR leads to autophosphorylation of the receptor and activation of the intrinsic tyrosine kinase activity, which allows appropriate substrates to be phosphorylated. Phosphorylation sites that are unique to each receptor presumably play a key role in these signaling differences. The catalytic loops within the tyrosine kinase domains contain a three tyrosine motif corresponding to Tyr1158, 1162 and 1163 (for the IR) and Tyr1131, 1135 and 1136 (for the IGF1R). Autophosphorylation of the tyrosine sites within the activation loop proceeds in a processive manner initiating at the second tyrosine (1162 or 1135), followed by phosphorylation at tyrosines 1158 (IGF-1R tyrosine 1131), 1163 (IGF-1R tyrosine 1136), resulting in the full activation of IR and IGF1R.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD220; CD221; IGF-I receptor; IGF-I Receptor beta; IGF1 Receptor; IGF1 Receptor beta; IGF1R beta; Insulin receptor; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; IR; soluble IGF1R variant 1; soluble IGF1R variant 2
基因别名: CD220; CD221; HHF5; IGF1R; IGFIR; IGFR; INSR; JTK13
UniProt ID: (Human) P08069, (Human) P06213
Entrez Gene ID: (Human) 3480, (Human) 3643
protein tyrosine kinase activity
insulin-like growth factor-activated receptor activity
insulin receptor binding
protein binding
insulin-like growth factor binding
ATP binding
insulin-like growth factor I binding
identical protein binding
phosphatidylinositol 3-kinase binding
insulin binding
insulin receptor substrate binding
receptor signaling protein tyrosine kinase activity
insulin-activated receptor activity
insulin-like growth factor receptor binding
GTP binding
protein phosphatase binding
lipoic acid binding
insulin-like growth factor II binding
protein complex binding
3-phosphoinositide-dependent protein kinase binding
PTB domain binding
transmembrane signal receptor
immune response
signal transduction
positive regulation of cell proliferation
insulin receptor signaling pathway
phosphatidylinositol 3-kinase signaling
positive regulation of cell migration
peptidyl-tyrosine autophosphorylation
negative regulation of apoptotic process
positive regulation of DNA replication
regulation of JNK cascade
protein autophosphorylation
insulin-like growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
protein tetramerization
inactivation of MAPKK activity
activation of MAPK activity
negative regulation of protein phosphorylation
positive regulation of protein phosphorylation
heart morphogenesis
carbohydrate metabolic process
regulation of transcription, DNA-templated
G-protein coupled receptor signaling pathway
epidermis development
male gonad development
response to manganese ion
regulation of hydrogen peroxide metabolic process
positive regulation of glycoprotein biosynthetic process
negative regulation of gene expression
response to activity
peptidyl-tyrosine phosphorylation
transformation of host cell by virus
signal transduction by protein phosphorylation
male sex determination
adrenal gland development
exocrine pancreas development
activation of protein kinase activity
activation of protein kinase B activity
response to estradiol
negative regulation of transporter activity
cellular response to insulin stimulus
response to vitamin D
response to testosterone
response to tumor necrosis factor
glucose homeostasis
positive regulation of MAPK cascade
positive regulation of nitric oxide biosynthetic process
fat cell differentiation
response to ethanol
positive regulation of glycogen biosynthetic process
positive regulation of glycolytic process
positive regulation of mitotic nuclear division
positive regulation of transcription, DNA-templated
regulation of embryonic development
positive regulation of glucose import
positive regulation of developmental growth
protein heterotetramerization
response to glucocorticoid
positive regulation of meiotic cell cycle
positive regulation of protein kinase B signaling
positive regulation of respiratory burst
cellular response to growth factor stimulus
regulation of female gonad development
