Invitrogen

Phospho-IkB alpha (Ser32, Ser36) Monoclonal Antibody (H.709.9)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

7个已发表图片

11 篇参考文献

查看其他41个IkB alpha抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

Phospho-IkB alpha (Ser32, Ser36) Antibody in Western Blot (WB)

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Phospho-IkB alpha (Ser32, Ser36) Antibody (MA5-15224) in WB

Western blot analysis of Phospho-I-kappa-B-alpha pSer32/36 in extracts from NIH/3T3 cells, untreated or TNF-alpha-treated (20 ng/mL) for 5 minutes, using Phospho-I-kappa-B-alpha pSer32/36 monoclonal antibody (Product # MA5-15224) (upper) or an I-kappa-B-alpha monoclonal antibody (Amino-terminal Antigen) (lower).

Phospho-IkB alpha (Ser32, Ser36) Antibody

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产品信息

MA5-15224

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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流式细胞分析 (Flow)

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产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

Human, Mouse, Pig

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

H.709.9

抗原

Synthetic phosphopeptide corresponding to residues surrounding pSer32/36 of human IkB alpha

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

22 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_10981266

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

IkB-alpha is a 40 kDa protein that functions to inhibit NF- kappaB activity. The inhibition occurs via protein-protein interaction between I kappaB proteins and NF- kappaB dimers in the cytosol. The interaction of I kappa B-alpha with NF- kappaB masks the nuclear localization sequence of NF- kappaB, preventing NF- kappaB translocation to the nucleus. A variety of stimuli can activate gene expression by liberating NF- kappaB through the degradation of I kappaB alpha. These stimuli include the proinflammatory cytokines TNF- alpha and IL-1 beta, chemokines, PMA, growth factors, LPS, UV irradiation, viral infection, as well as various chemical and physical stresses. In humans, the gene is located on the q arm of chromosome 14. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase alpha (IKK alpha), previously designated CHUK, interacts with IkB-alpha and specifically phosphorylates IkB-alpha on the sites that trigger its degradation Serines 32 and 36. IKK alpha appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKK alpha is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKK alpha, IKK beta and IKK gamma, and each appear to make essential contributions to IkB phosphorylation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Primary cells from patients with adult T cell leukemia/lymphoma depend on HTLV-1 Tax expression for NF-κB activation and survival.

Blood cancer journal

Hleihel R,Skayneh H,de Thé H,Hermine O,Bazarbachi A

MA5-15224 was used in Flow cytometry/Cell sorting to demonstrate the critical role of continued Tax and IL-10 expression for the survival of primary ATL cells, highlighting their relevance as therapeutic targets.

Wed May 03 00:00:00 EDT 2023

Primary cells from patients with adult T cell leukemia/lymphoma depend on HTLV-1 Tax expression for NF-κB activation and survival.

Blood cancer journal

Hleihel R,Skayneh H,de Thé H,Hermine O,Bazarbachi A

Wed May 03 00:00:00 EDT 2023

β-defensin 118 attenuates inflammation and injury of intestinal epithelial cells upon enterotoxigenic Escherichia coli challenge.

BMC veterinary research

Fu Q,Lin Q,Chen D,Yu B,Luo Y,Zheng P,Mao X,Huang Z,Yu J,Luo J,Yan H,He J

MA5-15224 was used in Western Blotting to show that DEFB118 pretreatment significantly increased the cell viability (P0.05) and decreased the expressions of inflammatory cytokines such as the interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) in IPEC-J2 cells exposure to ETEC (P0.05).

Tue Apr 19 00:00:00 EDT 2022

Immune-Stimulatory Effects of Curcumin on the Tumor Microenvironment in Head and Neck Squamous Cell Carcinoma.

Cancers

Kötting C,Hofmann L,Lotfi R,Engelhardt D,Laban S,Schuler PJ,Hoffmann TK,Brunner C,Theodoraki MN

Published figure using Phospho-IkB alpha (Ser32, Ser36) monoclonal antibody (Product # MA5-15224) in Western Blot

Tue Mar 16 00:00:00 EDT 2021

Interleukin-1α Is a Critical Mediator of the Response of Human Bronchial Fibroblasts to Eosinophilic Inflammation.

Cells

Bernau K,Leet JP,Floerke H,Bruhn EM,Noll AL,McDermott IS,Esnault S,Jarjour NN,Sandbo N

MA5-15224 was used in Western Blotting to determine key component(s) of eosinophil soluble factors that mediate IL6 and IL8 induction in human bronchial fibroblasts.

Tue Mar 02 00:00:00 EST 2021

Interleukin-1α Is a Critical Mediator of the Response of Human Bronchial Fibroblasts to Eosinophilic Inflammation.

Cells

Bernau K,Leet JP,Floerke H,Bruhn EM,Noll AL,McDermott IS,Esnault S,Jarjour NN,Sandbo N

MA5-15224 was used in Western Blotting to determine key component(s) of eosinophil soluble factors that mediate IL6 and IL8 induction in human bronchial fibroblasts.

Tue Mar 02 00:00:00 EST 2021

The Preeclamptic Environment Promotes the Activation of Transcription Factor Kappa B by P53/RSK1 Complex in a HTR8/SVneo Trophoblastic Cell Line.

International journal of molecular sciences

Sakowicz A,Bralewska M,Pietrucha T,Figueras F,Habrowska-Górczyńska DE,Piastowska-Ciesielska AW,Gach A,Sakowicz B,Rybak-Krzyszkowska M,Huras H,Grzesiak M,Biesiada L

Published figure using Phospho-IkB alpha (Ser32, Ser36) monoclonal antibody (Product # MA5-15224) in Western Blot

Wed Sep 22 00:00:00 EDT 2021

Estradiol/GPER affects the integrity of mammary duct-like structures in vitro.

Scientific reports

Deng Y,Miki Y,Nakanishi A

Published figure using Phospho-IkB alpha (Ser32, Ser36) monoclonal antibody (Product # MA5-15224) in Western Blot

Tue Jan 28 00:00:00 EST 2020

Interferon-Induced Protein 44 Interacts with Cellular FK506-Binding Protein 5, Negatively Regulates Host Antiviral Responses, and Supports Virus Replication.

mBio

DeDiego ML,Nogales A,Martinez-Sobrido L,Topham DJ

MA5-15224 was used in Western Blotting to investigate if interferon (IFN)-induced protein 44 (IFI44) positively affects virus production and if it negatively modulates innate immune responses induced after viral infections.

Tue Aug 27 00:00:00 EDT 2019

Plastin 3 influences bone homeostasis through regulation of osteoclast activity.

Human molecular genetics

Neugebauer J,Heilig J,Hosseinibarkooie S,Ross BC,Mendoza-Ferreira N,Nolte F,Peters M,Hölker I,Hupperich K,Tschanz T,Grysko V,Zaucke F,Niehoff A,Wirth B

MA5-15224 was used in Western Blot to show that regulation of osteoclastogenesis and bone remodeling via the PLS3-NKRF-NFκB-NFATC1 axis unveils a novel possibility to counteract osteoporosis.

Sat Dec 15 00:00:00 EST 2018

Plastin 3 influences bone homeostasis through regulation of osteoclast activity.

Human molecular genetics

Neugebauer J,Heilig J,Hosseinibarkooie S,Ross BC,Mendoza-Ferreira N,Nolte F,Peters M,Hölker I,Hupperich K,Tschanz T,Grysko V,Zaucke F,Niehoff A,Wirth B

MA5-15224 was used in Western Blot to show that regulation of osteoclastogenesis and bone remodeling via the PLS3-NKRF-NFκB-NFATC1 axis unveils a novel possibility to counteract osteoporosis.

Sat Dec 15 00:00:00 EST 2018

An autocrine inflammatory forward-feedback loop after chemotherapy withdrawal facilitates the repopulation of drug-resistant breast cancer cells.

Cell death & disease

Jia D,Li L,Andrew S,Allan D,Li X,Lee J,Ji G,Yao Z,Gadde S,Figeys D,Wang L

MA5-15224 was used in Western Blotting to provide rovides new insights into the communication between autocrine niches and signaling pathways in the development of chemotherapy resistance and CSCs and also offers a tangible approach in breast cancer treatment.

Thu Jul 13 00:00:00 EDT 2017

Cardamonin reduces chemotherapy-enriched breast cancer stem-like cells in vitro and in vivo.

Oncotarget

Jia D,Tan Y,Liu H,Ooi S,Li L,Wright K,Bennett S,Addison CL,Wang L

MA5-15224 was used in western blot to elucidate the reduction of chemotherap-enriched breast cancer stem-like cells in vivo and in vitro due to cardamonin

Tue Jan 05 00:00:00 EST 2016

生物信息学

蛋白别名: I(Kappa)B(alpha); I-kappa-B-alpha; I-kappaBalpha; IkappaBalpha; IkB-alpha; IKBalpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells alpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells, alpha; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappa-B inhibitor alpha; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, alpha; nuclear factor of kappa light polyp gene enhancer in B-cell 1; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha; RL/IF-1

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基因别名: AI462015; IKBA; MAD-3; MAD3; NFKBI; NFKBIA; RL/IF-1

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UniProt ID: (Human) P25963, (Rat) Q63746, (Mouse) Q9Z1E3

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Entrez Gene ID: (Human) 4792, (Rat) 25493, (Mouse) 18035

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功能

protein binding transcription factor binding nuclear localization sequence binding enzyme binding heat shock protein binding ubiquitin protein ligase binding protein complex binding identical protein binding NF-kappaB binding

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