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Phospho-JAK1 (Tyr1034) Antibody (PA5-104554) in ICC/IF
Immunofluorescent analysis of Phospho-JAK1 (Tyr1034, Tyr1035) in lovo cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% saponin, blocked with 10% serum (45 min, 37°C) incubated with Phospho-JAK1 (Tyr1034, Tyr1035) polyclonal antibody (Product # PA5-104554) using a dilution of 1:400 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
产品信息
PA5-104554
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human JAK1(Accession P23458), corresponding to amino acid residues around phosphorylated Tyr1034.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
sequential chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2816029
产品详细信息
Antibody detects endogenous levels of JAK1 only when phosphorylated at Tyr1034, which site historically referenced as Tyr1022.
靶标信息
Janus kinase 1 (JAK1), is a protein tyrosine kinase that is involved in the interferon-alpha/beta and -gamma signal transduction pathways. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK1. JAK1 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. Upon ligand binding, JAK1 undergoes tyrosine phosphorylation and catalytic activation in an interdependent manner. Phosphorylation of tyrosine residues at positions 1022 and 1023 is believed to function in the activation of catalytic events.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: EC 2.7.10.2; JAK-1; Janus kinase 1; Janus protein tyrosine kinase 1; kinase Jak1; Tyrosine-protein kinase JAK1
基因别名: AA960307; BAP004; C130039L05Rik; JAK1; JAK1A; JAK1B; JTK3
UniProt ID: (Human) P23458
Entrez Gene ID: (Human) 3716, (Rat) 84598, (Mouse) 16451
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
Ras guanyl-nucleotide exchange factor activity
receptor binding
growth hormone receptor binding
protein binding
ATP binding
protein phosphatase binding
ubiquitin protein ligase binding
CCR5 chemokine receptor binding
metal ion binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
enzyme linked receptor protein signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
cell migration
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
cell differentiation
intracellular signal transduction
peptidyl-tyrosine autophosphorylation
interleukin-2-mediated signaling pathway
regulation of cell proliferation
innate immune response
response to antibiotic
protein autophosphorylation
MAPK cascade
protein phosphorylation
positive regulation of GTPase activity
interferon-gamma-mediated signaling pathway
regulation of interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
regulation of type I interferon-mediated signaling pathway
positive regulation of sprouting angiogenesis
phosphorylation
