Invitrogen

Phospho-MST1/MST2 (Thr183, Thr180) Polyclonal Antibody

Phospho-MST1/MST2 (Thr183, Thr180) Antibody in Western Blot (WB)

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Phospho-MST1/MST2 (Thr183, Thr180) Antibody (PA5-121335) in WB

Western blot analysis of Phospho-MST1/MST2 (Thr183, Thr180) in extracts of various cell lines. Samples were incubated with Phospho-MST1/MST2 (Thr183, Thr180) Polyclonal antibody (Product # PA5-121335) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Both NIH/3T3 cells and C6 cells were treated by Staurosporine (1 µM) at room temperature fo... View More

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产品信息

PA5-121335

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic phosphorylated peptide around T180 of human STK3/STK4STK3 (NP_0062722)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2.23 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.01% thimerosal

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2914907

产品详细信息

Positive test controls include: NIH/3T3, C6.

Immunogen sequence: RNTVI

靶标信息

STK3 and STK4 are stress-activated, pro-apoptotic kinases which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. They are key components of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1. Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: KB-1458E12.1; kinase responsive to stress 2; Mammalian STE20-like protein kinase 1; Mammalian STE20-like protein kinase 2; mammalian sterile 20-like 1; MST-1; MST-2; MST1/C STK4; protein kinase homolog; serine/threonine kinase 3 (STE20 homolog, yeast); serine/threonine kinase 3 (Ste20, yeast homolog); Serine/threonine-protein kinase 3; Serine/threonine-protein kinase 4; Serine/threonine-protein kinase Krs-1; Serine/threonine-protein kinase Krs-2; STE20-like kinase MST1; STE20-like kinase MST2; sterile 20-like kinase 1; Yeast Sps1/Ste20-related kinase 3

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基因别名: 0610042I06Rik; AI447339; AU020804; Kas-2; KRS1; KRS2; Mess1; MST; MST1; MST2; Mst3; Ste20; STK3; STK4; YSK3