Invitrogen

Phospho-Paxillin (Ser126) Polyclonal Antibody

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

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抗体检测数据 (4)
高级验证 (1)

Phospho-Paxillin (Ser126) Antibody in Immunocytochemistry (ICC/IF)

Phospho-Paxillin (Ser126) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-Paxillin (Ser126) Antibody (44-1022G) in ICC/IF

Immunofluorescence analysis of Phospho-Paxillin pSer126 was done on 70% confluent log phase NIH/3T3 cells treated with 50 ng of PDGF for 10 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Paxillin pSer126 Rabbit Polyclonal Antibody (Product ... View More

Phospho-Paxillin (Ser126) Antibody in Immunocytochemistry (ICC/IF)

Phospho-Paxillin (Ser126) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-Paxillin (Ser126) Antibody in Western Blot (WB)

Phospho-Paxillin (Ser126) Antibody in Western Blot (WB)

Phospho-Paxillin (Ser126) Antibody

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Phospho-Paxillin (Ser126) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Phospho-Paxillin pSer126 was done on 70% confluent log phase NIH/3T3 cells treated with 50 ng of PDGF for 10 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Paxillin pSer126 Rabbit Polyclonal Antibody (Product # 44-1022G) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing punctuated nuclear localization. Panel e is untreated cell with no signal. Panel f is a no primary antibody control. The images were captured at 60X magnification.

Phospho-Paxillin (Ser126) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-Paxillin (pSer126) showing staining in the cytoplasm of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- Phospho-Paxillin pSer126 Polyclonal Antibody (Product # 44-1022G) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Phospho-Paxillin (Ser126) Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), A549 treated with 100 ng/mL of HGF treatment for 10 minutes (lane 2), HepG2 (lane 3), and HepG2 with 100 ng/mL of HGF treatment for 10 minutes (Lane 4). The blots were probed with Anti-Paxillin (PS126) Rabbit Polyclonal Antibody (Product # 44-1022G, 1: 1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (1:1000 dilution). A 68 kDa band corresponding to Paxillin (PS126) was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Phospho-Paxillin (Ser126) Antibody in Western Blot (WB) — Peptide Competition: Lysates from RAW 264.7 cells untreated (1) or treated with 1 mg/mL LPS for 60 minutes (2-6) were incubated with the Paxillin (pS126) antibody.

Phospho-Paxillin (Ser126) Antibody — Modulation of expression of target protein by cell treatment demonstrates antibody specificity. Immunofluorescence analysis of Phospho-Paxillin (Ser126) using Phospho-Paxillin (Ser126) Rabbit Polyclonal Antibody (Product # 44-1022G) shows induction of phosphorylation in NIH/3T3 cells treated with PDGF. {TM}

产品信息

44-1022G

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化（石蜡） (IHC (P))

1:20

-

免疫细胞化学 (ICC/IF)

1:250

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免疫沉淀 (IP)

-

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产品规格

种属反应

Mouse, Chicken, Human

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human paxillin that contains serine 126. The sequence is conserved in mouse and chicken.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533553

靶标信息

Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Glycogen synthase kinase 3- and extracellular signal-regulated kinase-dependent phosphorylation of paxillin regulates cytoskeletal rearrangement.

Molecular and cellular biology

Cai X,Li M,Vrana J,Schaller MD

Sat Apr 01 00:00:00 EST 2006

Glycogen synthase kinase 3- and extracellular signal-regulated kinase-dependent phosphorylation of paxillin regulates cytoskeletal rearrangement.

Molecular and cellular biology

Cai X,Li M,Vrana J,Schaller MD

Sat Apr 01 00:00:00 EST 2006

Glycogen synthase kinase 3- and extracellular signal-regulated kinase-dependent phosphorylation of paxillin regulates cytoskeletal rearrangement.

Molecular and cellular biology

Cai X,Li M,Vrana J,Schaller MD

Sat Apr 01 00:00:00 EST 2006

Glycogen synthase kinase 3- and extracellular signal-regulated kinase-dependent phosphorylation of paxillin regulates cytoskeletal rearrangement.

Molecular and cellular biology

Cai X,Li M,Vrana J,Schaller MD

Sat Apr 01 00:00:00 EST 2006

Glycogen synthase kinase 3- and extracellular signal-regulated kinase-dependent phosphorylation of paxillin regulates cytoskeletal rearrangement.

Molecular and cellular biology

Cai X,Li M,Vrana J,Schaller MD

Sat Apr 01 00:00:00 EST 2006

Glycogen synthase kinase 3- and extracellular signal-regulated kinase-dependent phosphorylation of paxillin regulates cytoskeletal rearrangement.

Molecular and cellular biology

Cai X,Li M,Vrana J,Schaller MD

Sat Apr 01 00:00:00 EST 2006

生物信息学

蛋白别名: FLJ16691; hCG_1778014; PAX-1; PAXI; Paxillin; paxillin alpha; testicular tissue protein Li 134

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基因别名: AW108311; AW123232; Pax; PXN

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UniProt ID: (Human) P49023, (Mouse) Q8VI36, (Chicken) P49024

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Entrez Gene ID: (Human) 5829, (Mouse) 19303, (Chicken) 395832

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integrin binding protein binding beta-catenin binding zinc ion binding vinculin binding protein kinase binding receptor signaling complex scaffold activity protein complex binding metal ion binding BH4 domain binding transcription cofactor activity actin or actin-binding cytoskeletal protein

参与通路

muscle contraction cell adhesion cell-matrix adhesion signal transduction signal complex assembly epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway cellular response to reactive oxygen species vascular endothelial growth factor receptor signaling pathway growth hormone receptor signaling pathway activation of MAPK activity movement of cell or subcellular component cytoskeleton organization integrin-mediated signaling pathway regulation of cell shape peptidyl-tyrosine phosphorylation lamellipodium assembly substrate adhesion-dependent cell spreading positive regulation of protein kinase activity focal adhesion assembly branching morphogenesis of an epithelial tube response to muscle stretch endothelial cell migration regulation of nucleic acid-templated transcription