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Phospho-Profilin 1 (Ser138) Antibody (PA5-143752) in WB
Western blot of Phospho-Profilin 1 (Ser138) in Jurkat stimulated with calyculin A (100 nM) for 30 min (lanes 1-6). The blots were probed with anti-Profilin (a.a. 132-140) (lanes 1-3) or Phospho-Profilin 1 (Ser138) polyclonal antibody (Product # PA5-143752) (lanes 4-6). Both antibodies were used in the absence (lanes 1 & 4) or presence of unphosphorylated Profilin (Ser-138) (lanes 2 & 5) or phospho-Profilin ... View More
产品信息
PA5-143752
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Phospho-Profilin (Ser138) synthetic peptide (coupled to carrier protein) that includes amino acids surrounding Ser138 in human Profilin-1. This sequence is conserved in rat and mouse Profilin-1. The serine site has some homology to the conserved site (Ser138) in Profilin-2a and Profilin-2b, but the site is not conserved in Profiin-3 and Profilin-4.
偶联物
Unconjugated
形式
Liquid
浓度
~0.5 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA, 50% glycerol
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2942980
产品详细信息
This antibody was cross-adsorbed to unphosphorylated Profilin (Ser-138) then affinity purified using Profilin (Ser-138) peptide (without carrier). The antibody detects a 15 kDa band corresponding to the apparent molecular mass of Profilin-1 in human Jurkat and A431 cells, as well as rat PC12 cells. This reactivity is decreased by lambda phosphatase treatment, and is blocked by phospho-profilin (Ser-138) blocking peptide.
靶标信息
Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role in both signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Profilin I appears to be highly expressed in most tissues except for skeletal muscle. Profilin II is predominantly expressed in brain and at lower levels in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: actin binding protein; Epididymis tissue protein Li 184a; Profilin I; Profilin-1
基因别名: ALS18; Pfn; PFN1
UniProt ID: (Human) P07737, (Rat) P62963, (Mouse) P62962
Entrez Gene ID: (Human) 5216, (Rat) 64303, (Mouse) 18643
adenyl-nucleotide exchange factor activity
actin binding
actin monomer binding
receptor binding
protein binding
phosphatidylinositol-4,5-bisphosphate binding
Rho GTPase binding
poly(A) RNA binding
proline-rich region binding
cadherin binding involved in cell-cell adhesion
actin or actin-binding cytoskeletal protein
cytoskeletal protein
non-motor actin binding protein
neural tube closure
positive regulation of epithelial cell migration
actin cytoskeleton organization
negative regulation of actin filament polymerization
positive regulation of actin filament polymerization
negative regulation of actin filament bundle assembly
positive regulation of actin filament bundle assembly
positive regulation of ATPase activity
positive regulation of transcription from RNA polymerase II promoter
positive regulation of viral transcription
protein stabilization
positive regulation of DNA metabolic process
positive regulation of stress fiber assembly
negative regulation of stress fiber assembly
Wnt signaling pathway, planar cell polarity pathway
cellular response to growth factor stimulus
cell-cell adhesion
positive regulation of ruffle assembly
regulation of transcription from RNA polymerase II promoter
actin polymerization or depolymerization
response to organic substance
regulation of actin polymerization or depolymerization
sequestering of actin monomers
