Invitrogen

Phospho-RSK Pan (Ser221, Ser227, Ser218, Ser232) Polyclonal Antibody

Phospho-RSK Pan (Ser221, Ser227, Ser218, Ser232) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Phospho-RSK Pan (Ser221, Ser227, Ser218, Ser232) Antibody (PA5-114637) in IHC (P)

Immunohistochemistry analysis of Phospho-RSK1/RSK2/RSK3/RSK4 (Ser221, Ser227, Ser218, Ser232) in human ovarian cancer and adjacent normal tissues. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114637) at a dilution of 1:100. Secondary staining was applied with HRP conjugated ant... View More

Phospho-RSK Pan (Ser221, Ser227, Ser218, Ser232) Antibody in Western Blot (WB)

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产品信息

PA5-114637

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human RSK1/2/3/4 around the phosphorylation site of Ser221/227/s218/232.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

sequential chromatography

保存液

PBS with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2899273

产品详细信息

Antibody detects endogenous levels of RSK1/2/3/4 only when phosphorylated at Ser221/227/s218/232.

靶标信息

The p90 ribosomal S6 kinases (RSKs) comprise a family of serine/threonine kinases that lie at the terminus of the ERK pathway. In humans, the RSK family consists of four isoforms (RSK1 to-4). RSK family members are unusual among serine/threonine kinases in that they contain two distinct kinase domains, both of which are catalytically functional. Theses kinase domains are activated in a sequential manner by a series of phosphorylations. RSK regulates gene expression via association and phosphorylation of transcriptional regulators including c-Fos, estrogen receptor, NFkappaB/IkappaB alpha, cAMP-response element-binding protein (CREB). ERK activates the C-terminal kinase of RSK, leading to activation of the N-terminal kinase. Members of the RSK family are present in the cytoplasm as well as the nucleus. Addition of growth factor to the cells results in the activation of both cytosolic and nuclear RSK and the translocation of the cytosolic RSK into the nucleus upon activation. The activation and nuclear translocation of RSK result in phosphorylation and activation of transcription factors.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 90 kDa ribosomal protein S6 kinase 1; 90 kDa ribosomal protein S6 kinase 2; 90 kDa ribosomal protein S6 kinase 3; 90 kDa ribosomal protein S6 kinase 4; 90 kDa ribosomal protein S6 kinase 6; coiled-coil domain containing 88B; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); Insulin-stimulated protein kinase 1; ISPK-1; MAP kinase-activated protein kinase 1a; MAP kinase-activated protein kinase 1b; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1a; MAPK-activated protein kinase 1b; MAPK-activated protein kinase 1c; MAPKAP kinase 1a; MAPKAP kinase 1b; MAPKAP kinase 1C; MAPKAPK-1a; MAPKAPK-1b; MAPKAPK-1c; MAPKAPK1A; MAPKAPK1D; mitogen- and stress-activated protein kinase 2; mitogen- and stress-activated protein kinase-2; mitogen-activated protein kinase-activated protein kinase 1C; Nuclear mitogen- and stress-activated protein kinase 2; p90 rsk; p90-RSK 1; p90-RSK 2; p90-RSK 3; p90-RSK 6; p90RSK1; p90RSK2; p90RSK6; p90S6K; pp90RSK1; pp90RSK2; pp90RSK3; pp90RSK4; Protein-tyrosine kinase Mpk-9; Ribosomal protein kinase B; ribosomal protein S6 kinase 2; ribosomal protein S6 kinase alpha 1; ribosomal protein S6 kinase alpha 2; ribosomal protein S6 kinase alpha 4; Ribosomal protein S6 kinase alpha-1; Ribosomal protein S6 kinase alpha-2; Ribosomal protein S6 kinase alpha-3; Ribosomal protein S6 kinase alpha-4; Ribosomal protein S6 kinase alpha-6; ribosomal protein S6 kinase polypeptide 1; ribosomal protein S6 kinase, 90kD, polypeptide 1; Ribosomal protein S6 kinase, 90kD, 1; ribosomal protein S6 kinase, 90kD, polypeptide 2; ribosomal protein S6 kinase, 90kD, polypeptide 4; ribosomal protein S6 kinase, 90kDa, polypeptide 1; ribosomal protein S6 kinase, 90kDa, polypeptide 2; ribosomal protein S6 kinase, 90kDa, polypeptide 3; ribosomal protein S6 kinase, 90kDa, polypeptide 4; ribosomal protein S6 kinase, 90kDa, polypeptide 6; Ribosomal S6 kinase 1; Ribosomal S6 kinase 2; Ribosomal S6 kinase 3; Ribosomal S6 kinase 4; RLSK; RP11-393H10.3; RSK-1; RSK-2; RSK-3; RSK-4; RSK-like protein kinase; RSKB; S6 protein kinase (Rsk-1); S6K-alpha 1; S6K-alpha 2; S6K-alpha 6; S6K-alpha-1; S6K-alpha-2; S6K-alpha-3; S6K-alpha-4; S6K-alpha-6

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基因别名: 1110069D02Rik; 2610524K04Rik; 90kDa; AI848992; C130006E23; Ccdc88b; CLS; D17Wsu134e; HU-1; HU-2; HU-3; ISPK-1; ISPK1; Mapkapk-1a; MAPKAPK-1b; MAPKAPK1A; MAPKAPK1B; MAPKAPK1C; mMSK2; MPK-9; MRX19; MSK2; p90-Rsk1; p90-RSK2; p90-RSK3; p90Rsk; p90Rsk1; p90RSK2; p90RSK3; p90S6K; pp90rsk; pp90RSK2; pp90RSK3; PP90RSK4; RGD1560817; RGD1563860; Rps6ka-rs1; RPS6KA1; RPS6KA2; RPS6KA3; RPS6KA4; RPS6KA6; RSK; Rsk-1; RSK-B; RSK1; RSK2; RSK3; RSK4; S6K-alpha; S6K-alpha-1; S6K-alpha-4; S6K-alpha-6; S6K-alpha2; S6K-alpha3