Invitrogen

Phospho-STAT5A/B (Tyr694) Polyclonal Antibody

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抗体检测数据 (4)

Phospho-STAT5A/B (Tyr694) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-STAT5A/B (Tyr694) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Phospho-STAT5A/B (Tyr694) Antibody (PA5-121312) in IHC (P)

Immunohistochemistry analysis of Phospho-STAT5A/B (Tyr694) in paraffin-embedded human placenta. Samples were incubated with Phospho-STAT5A/B (Tyr694) Polyclonal antibody (Product # PA5-121312) using a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Phospho-STAT5A/B (Tyr694) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-STAT5A/B (Tyr694) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-STAT5A/B (Tyr694) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-STAT5A/B (Tyr694) Antibody in Western Blot (WB)

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Phospho-STAT5A/B (Tyr694) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-STAT5A/B (Tyr694) in paraffin-embedded human placenta. Samples were incubated with Phospho-STAT5A/B (Tyr694) Polyclonal antibody (Product # PA5-121312) using a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Phospho-STAT5A/B (Tyr694) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-STAT5A/B (Tyr694) in paraffin-embedded mouse liver. Samples were incubated with Phospho-STAT5A/B (Tyr694) Polyclonal antibody (Product # PA5-121312) using a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Phospho-STAT5A/B (Tyr694) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-STAT5A/B (Tyr694) in paraffin-embedded rat heart. Samples were incubated with Phospho-STAT5A/B (Tyr694) Polyclonal antibody (Product # PA5-121312) using a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Phospho-STAT5A/B (Tyr694) Antibody in Western Blot (WB) — Western blot analysis of Phospho-STAT5A/B (Tyr694) in NIH/3T3 cells. Samples were incubated with Phospho-STAT5A/B (Tyr694) Polyclonal antibody (Product # PA5-121312) using a dilution of 1:400, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. NIH/3T3 cells were treated by Pervanadate (1 nM) at 37°C for 30 minutes after serum-starvation overnight. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

产品信息

PA5-121312

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:500

-

免疫组化（石蜡） (IHC (P))

1:100-1:200

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic phosphorylated peptide around Y694 of human STAT5 (NP_003143.2/NP_036580.2).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.45 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.05% ProClin 300

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2914884

产品详细信息

Positive test controls include: K562.

Immunogen sequence: DGYVK

靶标信息

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor-associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of, many cell ligands, such as IL-2, IL-3, IL-7, GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/ BCL-X(L), which suggests the antiapoptotic function of this gene in cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Mammary gland factor; mammary gland factor STAT5A; mammary gland factor STAT5B; signal transducer and activator of transcription 5; Signal transducer and activator of transcription 5A; Signal transducer and activator of transcription 5B; STA5B; STAT; transcription factor STAT5B

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基因别名: AA959963; MGF; Mpf; STAT5; STAT5A; STAT5B

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UniProt ID: (Human) P42229, (Human) P51692, (Rat) Q62771, (Mouse) P42230, (Rat) P52632, (Mouse) Q541Q5

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Entrez Gene ID: (Human) 6776, (Human) 6777, (Rat) 24918, (Mouse) 20850, (Rat) 25126, (Mouse) 20851

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DNA binding transcription factor activity, sequence-specific DNA binding protein tyrosine kinase activity signal transducer activity protein binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding protein phosphatase binding glucocorticoid receptor binding protein dimerization activity double-stranded DNA binding sequence-specific DNA binding DNA-binding transcription factor

参与通路

allantoin metabolic process positive regulation of endothelial cell proliferation citrate metabolic process 2-oxoglutarate metabolic process succinate metabolic process oxaloacetate metabolic process transcription, DNA-templated regulation of transcription from RNA polymerase II promoter isoleucine metabolic process valine metabolic process creatine metabolic process fatty acid metabolic process JAK-STAT cascade lactation peptidyl-tyrosine phosphorylation taurine metabolic process prolactin signaling pathway regulation of multicellular organism growth positive regulation of blood vessel endothelial cell migration creatinine metabolic process JAK-STAT cascade involved in growth hormone signaling pathway luteinization response to hypoxia natural killer cell differentiation liver development transcription from RNA polymerase II promoter acute-phase response female pregnancy regulation of steroid metabolic process lipid storage regulation of epithelial cell differentiation response to estradiol response to lipopolysaccharide positive regulation of natural killer cell proliferation positive regulation of natural killer cell differentiation cellular response to hormone stimulus T cell differentiation in thymus positive regulation of multicellular organism growth positive regulation of activated T cell proliferation progesterone metabolic process T cell homeostasis negative regulation of apoptotic process positive regulation of interleukin-2 biosynthetic process response to ethanol positive regulation of B cell differentiation positive regulation of gamma-delta T cell differentiation negative regulation of erythrocyte differentiation positive regulation of erythrocyte differentiation positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter positive regulation of natural killer cell mediated cytotoxicity development of secondary female sexual characteristics development of secondary male sexual characteristics Peyer's patch development positive regulation of smooth muscle cell proliferation positive regulation of inflammatory response positive regulation of cellular component movement response to interleukin-2 response to interleukin-4 response to interleukin-15 cellular response to growth factor stimulus cellular response to epidermal growth factor stimulus mast cell migration regulation of transcription, DNA-templated signal transduction positive regulation of cell proliferation cytokine-mediated signaling pathway regulation of cell adhesion mammary gland development response to nutrient levels negative regulation of mast cell apoptotic process response to peptide hormone positive regulation of lymphocyte differentiation multicellular organismal reproductive process mammary gland involution positive regulation of mast cell differentiation prostate gland epithelium morphogenesis epithelial cell differentiation involved in prostate gland development mammary gland epithelium development positive regulation of mast cell proliferation cellular response to granulocyte macrophage colony-stimulating factor stimulus sex differentiation