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Phospho-TSC1 (Ser505) Antibody (PA5-105060) in IHC (P)
Immunohistochemistry analysis of Phospho-TSC1 (Ser505) in rat pancreatic tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-TSC1 (Ser505) polyclonal antibody (Product # PA5-105060) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
产品信息
PA5-105060
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human TSC1(Accession Q92574), corresponding to amino acid residues around phosphorylated Ser505.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
sequential chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2816533
产品详细信息
Antibody detects endogenous levels of TSC1 only when phosphorylated at Ser505.
靶标信息
Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Hamartin; HGNC:12362; MGC86987; OTTHUMP00000022439; OTTHUMP00000198378; Tuberous sclerosis 1 protein; Tuberous sclerosis 1 protein homolog; tumor suppressor
基因别名: KIAA0243; LAM; TSC; TSC1
UniProt ID: (Human) Q92574, (Rat) Q9Z136, (Mouse) Q9EP53
Entrez Gene ID: (Human) 7248, (Rat) 60445, (Mouse) 64930
kidney development
neural tube closure
regulation of cell-matrix adhesion
adaptive immune response
rRNA export from nucleus
regulation of translation
potassium ion transport
cell cycle arrest
cell-matrix adhesion
negative regulation of cell proliferation
adult locomotory behavior
negative regulation of macroautophagy
negative regulation of translation
hippocampus development
cerebral cortex development
cell projection organization
negative regulation of TOR signaling
response to insulin
myelination
memory T cell differentiation
regulation of phosphoprotein phosphatase activity
negative regulation of cell size
regulation of protein kinase activity
glucose import
negative regulation of insulin receptor signaling pathway
synapse organization
protein stabilization
protein heterooligomerization
regulation of stress fiber assembly
regulation of cell cycle
positive regulation of focal adhesion assembly
cardiac muscle cell differentiation
activation of GTPase activity
cellular response to oxygen-glucose deprivation
regulation of neuron death
nervous system development
regulation of actin cytoskeleton organization
regulation of focal adhesion assembly
embryo development
regulation of GTPase activity
