Invitrogen

Phospho-Tau (Thr181) Recombinant Rabbit Monoclonal Antibody (5H9L11)

Phospho-Tau (Thr181) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Phospho-Tau (Thr181) Antibody (701530) in IHC (P)

Immunohistochemistry analysis of Phospho-Tau pThr181 showing staining in the cytoplasm of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room t... View More

Phospho-Tau (Thr181) Antibody in Western Blot (WB)

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产品信息

701530

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:5,000

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免疫组化（石蜡） (IHC (P))

1:10-1:100

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

5H9L11

抗原

human Tau around phosphorylated Tyrosine 181.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2532491

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Aberrant palmitoylation caused by a ZDHHC21 mutation contributes to pathophysiology of Alzheimer's disease.

BMC medicine

Li W,Pang Y,Wang Y,Mei F,Guo M,Wei Y,Li X,Qin W,Wang W,Jia L,Jia J

701530 was used in Western Blotting to suggest that aberrant protein palmitoylation mediated by ZDHHC21 mutations is a new pathogenic mechanism of Alzheimer's disease, warranting further investigations for the development of therapeutic interventions.

Mon Jun 26 00:00:00 EDT 2023

Histone H2A ubiquitination resulting from Brap loss of function connects multiple aging hallmarks and accelerates neurodegeneration.

iScience

Guo Y,Chomiak AA,Hong Y,Lowe CC,Kopsidas CA,Chan WC,Andrade J,Pan H,Zhou X,Monuki ES,Feng Y

701530 was used in Western Blot to demonstrate how abrogation of the Brap BRCA-1 associated protein for neurogenesis results in persistent DNA double-strand breaks and elevation of histone H2A mono- and poly-ubiquitination (H2Aub) which may act as a nexus multiple aging hallmarks and promote tissue-wide degeneration.

Fri Jul 15 00:00:00 EDT 2022

Nde1 is required for heterochromatin compaction and stability in neocortical neurons.

iScience

Chomiak AA,Guo Y,Kopsidas CA,McDaniel DP,Lowe CC,Pan H,Zhou X,Zhou Q,Doughty ML,Feng Y

701530 was used in Immunohistochemistry (Paraffin), Western Blot to demonstrate that the Nde1 gene controls neurogenesis through facilitating H4K20 trimethylation-mediated heterochromatin compaction.

Fri Jun 17 00:00:00 EDT 2022

Opposing effects of progranulin deficiency on amyloid and tau pathologies via microglial TYROBP network.

Acta neuropathologica

Takahashi H,Klein ZA,Bhagat SM,Kaufman AC,Kostylev MA,Ikezu T,Strittmatter SM

701530 was used in western blot to see that the granulin Alzheimer's disease risk variant has no significant effects on florbetapir positron emission tomographic amyloid imaging and cerebrospinal fluid Abeta levels

Mon May 01 00:00:00 EDT 2017

生物信息学

蛋白别名: FLJ31424; FTDP17; G protein beta1/gamma2 subunit-interacting factor 1; map tau; MGC138549; Microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; microtubules; Neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; Paired helical filament-tau; PHF-tau; PHFtau; protein phosphatase 1, regulatory subunit 103; Tau microtubule-associated protein; Tau-4; Tau5

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基因别名: AI413597; AW045860; DDPAC; FTDP-17; MAPT; MAPTL; MSTD; Mtapt; MTBT1; MTBT2; PPND; PPP1R103; pTau; RNPTAU; TAU

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UniProt ID: (Human) P10636, (Mouse) P10637

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Entrez Gene ID: (Human) 4137, (Rat) 29477, (Mouse) 17762

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功能