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Phospho-ULK1 (Ser556) Antibody (80218-1-RR100UL) in WB
Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with (Product # 80218-1-RR100UL) (Phospho-ULK1 (Ser556) antibody) at dilution of 1:5,000 incubated at room temperature for 1.5 hours. The membrane was tripped and re-blotted with Mouse anti-mTOR antibody 66888-1-Ig as loading control.
产品信息
80218-1-RR100UL
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
proprietary
分类
Recombinant Monoclonal
类型
Antibody
克隆号
2F5
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
靶标信息
ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: APG1; ATG 1; ATG1; ATG1 autophagy related 1 homolog; Autophagy-related protein 1 homolog; C. elegans; EC:2.7.11.1; FLJ38455; Serine/threonine-protein kinase ULK1; Serine/threonine-protein kinase Unc51.1; ULK 1; UNC 51; Unc-51-like kinase 1
基因别名: ATG1; ATG1A; AU041434; hATG1; KIAA0722; mKIAA0722; ULK1; UNC51; Unc51.1
UniProt ID: (Human) O75385, (Mouse) O70405
Entrez Gene ID: (Human) 8408, (Mouse) 22241
protein serine/threonine kinase activity
protein binding
ATP binding
Rab GTPase binding
protein complex binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein kinase binding
Hsp90 protein binding
non-receptor serine/threonine protein kinase
protein phosphorylation
signal transduction
protein localization
regulation of autophagy
positive regulation of autophagy
macroautophagy
positive regulation of macroautophagy
peptidyl-serine phosphorylation
neuron projection regeneration
neuron projection development
negative regulation of protein complex assembly
cellular response to nutrient levels
response to starvation
protein autophosphorylation
axon extension
autophagosome assembly
autophagy
Ras protein signal transduction
axonogenesis
regulation of gene expression
regulation of cell death
phosphorylation
cerebellar granule cell differentiation
radial glia guided migration of cerebellar granule cell
receptor internalization
negative regulation of collateral sprouting
regulation of neurotrophin TRK receptor signaling pathway
mitophagy in response to mitochondrial depolarization
