Invitrogen

Phospho-Vinculin (Tyr822) Polyclonal Antibody

Phospho-Vinculin (Tyr822) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Phospho-Vinculin (Tyr822) Antibody (44-1080G) in IHC (P)

Immunohistochemistry analysis of Phospho-Vinculin (pTyr822) showing staining in the cytoplasm of paraffin-embedded mouse heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at ... View More

Phospho-Vinculin (Tyr822) Antibody in Western Blot (WB)

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产品信息

44-1080G

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化（石蜡） (IHC (P))

1:10-1:100

产品规格

种属反应

Chicken, Human, Mouse, Rat

已发表种属

Human, Rat, Tag

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human vinculin that contains tyrosine 822. The sequence is conserved in mouse, rat, and chicken.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533569

靶标信息

Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Adhesion molecules affected by treatment of lung cancer cells with epidermal growth factor.

Lung

Fonseca FL,Azzalis LA,Feder D,Nogoceke E,Junqueira VB,Valenti VE,de Abreu LC

Sat Oct 01 00:00:00 EDT 2011

Adhesion molecules affected by treatment of lung cancer cells with epidermal growth factor.

Lung

Fonseca FL,Azzalis LA,Feder D,Nogoceke E,Junqueira VB,Valenti VE,de Abreu LC

Sat Oct 01 00:00:00 EDT 2011

Adhesion molecules affected by treatment of lung cancer cells with epidermal growth factor.

Lung

Fonseca FL,Azzalis LA,Feder D,Nogoceke E,Junqueira VB,Valenti VE,de Abreu LC

Sat Oct 01 00:00:00 EDT 2011

The Helicobacter pylori CagA protein disrupts matrix adhesion of gastric epithelial cells by dephosphorylation of vinculin.

Cellular microbiology

Moese S,Selbach M,Brinkmann V,Karlas A,Haimovich B,Backert S,Meyer TF

Tue May 01 00:00:00 EDT 2007

生物信息学

蛋白别名: epididymis luminal protein 114; meta-vinculin; Metavinculin; metavinculin {ECO:0000250|UniProtKB:P18206}; MV; RP11-178G16.3; unnamed protein product; vcl {ECO:0000250|UniProtKB:P18206}; vinc; vinc 1; Vinculin; vinculin {ECO:0000250|UniProtKB:P18206}

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基因别名: 9430097D22; AA571387; AI462105; AW545629; CMD1W; CMH15; HEL114; MV; MVCL; VCL; VINC1