Invitrogen

Phospho-YAP1 (Ser128) Polyclonal Antibody

Phospho-YAP1 (Ser128) Antibody in Western Blot (WB)

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Phospho-YAP1 (Ser128) Antibody (PA5-117264) in WB

Western blot analysis of Phospho-YAP1 (Ser128) in extracts of NIH/3T3 cells. Samples were incubated with Phospho-YAP1 (Ser128) Polyclonal antibody (Product # PA5-117264) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. NIH/3T3 cells were treated by Forskolin (30 µM) at 37°C for 30 minutes after serum-starvation overnight. Lysates/proteins:... View More

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产品信息

PA5-117264

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1000-1:5000

产品规格

种属反应

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic phosphorylated peptide around S128 of human YAP1 (NP_0011236171)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2.1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.05% ProClin 300

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2901894

产品详细信息

Positive Samples: NIH/3T3

Immunogen sequence: HSSPA

靶标信息

YAP (Yes-Associated Protein) is a founding member of the WW domain family of proteins, transcriptional co-activator and oncogene. Its gene is transcriptionally activated in squamous cell carcinoma cells. YAP can act both as a coactivator and corepressor and is the critical downstream regulatory target in the Hippo signaling pathway. The Hippo signaling pathway plays a pivotal role in organ size control and tumour suppression by restricting proliferation and promoting apoptosis. Also plays key roles in tissue tension, 3D tissue shape, cell proliferation, cell death, and cell migration. Mutations in the IRF5 gene results in coloboma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 65 kDa Yes-associated protein; Protein yorkie homolog; Transcriptional coactivator YAP1; Yes-associated protein 1; Yes-associated protein YAP65 homolog; yes-associated protein, 65 kDa; yorkie homolog

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基因别名: AI325207; Yap; Yap1; Yap65; Yki; Yorkie

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UniProt ID: (Mouse) P46938

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Entrez Gene ID: (Mouse) 22601

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功能

RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor binding chromatin binding transcription coactivator activity transcription corepressor activity protein binding protein C-terminus binding transcription regulatory region DNA binding proline-rich region binding transcription cofactor

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