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Phospho-Zap-70 (Tyr319, Tyr352) Antibody (MA5-35925) in WB
Western Blot analysis of Phospho-Zap-70 in untreated (–) and treated (+) Jurkat whole cell extract. Sample was separated with 7.5% SDS-PAGE, incubated with monoclonal antibody (Product # MA5-35925) at a dilution of 1:1,000, and followed by HRP-conjugated anti-mouse IgG.
产品信息
MA5-35925
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
GT364
抗原
Carrier-protein conjugated synthetic peptide corresponding to residues around human ZAP70 (phospho Tyr319)/Syk (phospho Tyr352). The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2866542
靶标信息
ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; EC 2.7.10.2; FLJ17670; FLJ17679; kinase ZAP70; Selective T cell defect; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; Tyrosine-protein kinase ZAP-70;TZK; ZA70; Zeta chain associated protein kinase 70kDa; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
基因别名: ADMIO2; IMD48; SRK; STCD; STD; TZK; ZAP-70; ZAP70
UniProt ID: (Human) P43403
Entrez Gene ID: (Human) 7535
adaptive immune response
macrophage activation involved in immune response
neutrophil activation involved in immune response
protein phosphorylation
inflammatory response
immune response
transmembrane receptor protein tyrosine kinase signaling pathway
peptidyl-tyrosine phosphorylation
T cell differentiation
positive regulation of cell adhesion mediated by integrin
intracellular signal transduction
peptidyl-tyrosine autophosphorylation
T cell activation
B cell activation
positive regulation of mast cell degranulation
beta selection
positive thymic T cell selection
negative thymic T cell selection
innate immune response
positive regulation of B cell differentiation
positive regulation of T cell differentiation
positive regulation of alpha-beta T cell differentiation
positive regulation of alpha-beta T cell proliferation
positive regulation of calcium-mediated signaling
T cell receptor signaling pathway
B cell receptor signaling pathway
T cell aggregation
T cell migration
regulation of platelet aggregation
