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Invitrogen
Podocin Polyclonal Antibody
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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Podocin Antibody (PA5-79757) in IHC (P)
Immunohistochemical analysis of NPHS2 in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-NPHS2 antibody (Product # PA5-79757) overnight at 4°C. Biotinylated goat anti-rabbit IgG was ... View More
产品信息
PA5-79757
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the C-terminus of human NPHS2 (368-383aa KPVEPLNPKKKDSPML).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide, 0.05mg thimerosal
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2746872
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
靶标信息
The pathological conditions of SRN1 correlate well with mutations at the NPHS2 gene, where expression of a protein known as Podocin occurs. Abnormal or inefficient signaling through Podocin protein-dependent networks contributes to the development of podocyte dysfunction and proteinuria. The human NPHS2 gene maps to chromosome 1q25-q31 and encodes a 383 amino acid protein. Podocin is an integral membrane protein that appears to fold into a hairpin-like structure with intracellular amino- and carboxy-termini. Transmembrane and cytoplasmic portions of Podocin share homology to the corresponding regions of the stomatin family proteins. Expression of high-order oligomers of Podocin in glomerular podocytes may reflect a scaffolding function that influences proper function of the glomerular filtration barrier, which is necessary for renal stability.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: nephrosis 2 homolog, podocin; nephrosis 2 idiopathic steroid-resistant (podocin); nephrosis 2, idiopathic, steroid-resistant (podocin); NPHS2 podocin; Podocin
基因别名: AI790225; NPHS2; PDCN; podocin; SRN1
UniProt ID: (Human) Q9NP85, (Mouse) Q91X05, (Rat) Q8K4G9
Entrez Gene ID: (Human) 7827, (Mouse) 170484, (Rat) 170672
