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Invitrogen
Pokemon (LRF) Monoclonal Antibody (13E9), eFluor™ 660, eBioscience™
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Pokemon (LRF) Antibody (50-3309-80) in IHC (P)
Immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue using 5 µg/mL Armenian Hamster IgG Isotype Control eFluor® 660 (left) or 5 µg/mL Anti-Human Pokeman (LRF) eFluor® 660 (right). Nuclei are stained with DAPI (colocalization appears pink).
产品信息
50-3309-80
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Mouse
宿主/亚型
Armenian hamster
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
13E9
偶联物
激发/发射光谱
651/668 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2574200
产品详细信息
Description: This monoclonal antibody reacts with human Leukemia/lymphoma-related factor (LRF). This 65-kDa protein is also known as POKEMON, FBI-1, and Zbtb7a. LRF is a member of the POK family of transcriptional repressors, and is expressed in hematopoietic stem cells, T and B lymphocytes, macrophages, and erythroid cells. This protein is also highly expressed in human non-Hodgkin's lymphoma tissues and diffuse large B-cell lymphomas, demonstrating its prominent role in oncogenic transformation. Studies have demonstrated that LRF is also involved in B cell lineage commitment and development by opposing Notch signaling. Moreover, LRF is critical for erythroid terminal differentiation in the bone marrow by inhibiting Bim-mediated apoptosis. Finally, LRF interacts with Bcl-6 in germinal center B cells.
Applications Reported: This 13E9 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.
Applications Tested: This 13E9 antibody has been tested by immunohistochemistry on human FFPE tissue using high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Plays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals. Specifically represses the transcription of the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3'.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DKFZp547O146; Factor binding IST protein 1; Factor that binds to inducer of short transcripts protein 1; FBI-1; HIV-1 1st-binding protein 1; HIV-1 inducer of short transcripts binding protein; Leukemia/lymphoma-related factor; lymphoma related factor; MGC99631; POK erythroid myeloid ontogenic factor; POZ and Krueppel erythroid myeloid ontogenic factor; TIP21; TTF-I-interacting peptide 21; zi; zinc finger and BTB domain containing 7A, HIV-1 inducer of short transcripts binding protein; Zinc finger and BTB domain-containing protein 7A; Zinc finger protein 857A
基因别名: FBI-1; FBI1; LRF; pokemon; TIP21; ZBTB7; ZBTB7A; ZNF857A
UniProt ID: (Human) O95365
Entrez Gene ID: (Human) 51341
