Invitrogen

Prolactin Receptor Monoclonal Antibody (A6E4)

Prolactin Receptor Antibody in Immunocytochemistry (ICC/IF)

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Prolactin Receptor Antibody (MA5-50553) in ICC/IF

Immunocytochemistry analysis of Prolactin Receptor in MCF-7 cells. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were incubated with Prolactin Receptor monoclonal antibody (Product # MA5-50553) with a dilution of 1:100 in 2% BSA overnight at 4 . Followed by secondary a... View More

Prolactin Receptor Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Prolactin Receptor Antibody in Western Blot (WB)

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产品信息

MA5-50553

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

免疫组化（石蜡） (IHC (P))

1:200

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

A6E4

抗原

Recombinant protein within Human Prolactin Receptor aa 150-350/622.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 0.05% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_3091131

产品详细信息

Positive control: 293T cell lysate, Hela cell lysate, MCF-7, human kidney tissue, human pancreas tissue.

Predicted band size: 70 kDa

Subcellular Location: Membrane, Secreted.

靶标信息

Prolactin (PRL) is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor (PRLR), a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. PRLR consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

仅用于科研。不用于诊断过程。未经明确授权不得转售。