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Prolactin Receptor Antibody (MA1-611) in ICC/IF
Immunofluorescent analysis of Prolactin Receptor in H-4-II-E Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Prolactin Receptor monoclonal antibody (Product # MA1-611) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Prolactin Recept... View More
产品信息
MA1-611
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Rat
已发表种属
Human,
Rat
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
T6
抗原
Purified rat liver PRL receptor.
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2171289
产品详细信息
MA1-611 detects prolactin (PRL) receptor in rat and human tissues. This antibody does not cross-react with PRL receptors from other species nor with growth hormone (GH) receptor.
MA1-611 has been successfully used in Western blot, IF, immunohistochemistry (4% PFA fixed tissue), and immunocytochemistry procedures. By Western blot, this antibody detects an ~42 kDa protein representing PRL receptor in NB2 cell lysate. Immunohistochemical staining of PRL receptor in NB2 cells with MA1-611 yields a staining pattern consistent with cytoplasmic vesicular staining. MA1-611 has also been used to inhibit the binding of prolactin to prolactin receptor in vitro.
The MA1-611 immunogen is purified rat liver PRL receptor.
Reconstitute with 100 µL PBS.
靶标信息
Prolactin (PRL) is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor (PRLR), a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. PRLR consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: hPRL receptor; Lactogen receptor; Prl R; PRL-R; Prolactin receptor; secreted prolactin binding protein
基因别名: HPRL; hPRLrI; MFAB; PRLR; RATPRLR
UniProt ID: (Human) P16471, (Rat) P05710
Entrez Gene ID: (Human) 5618, (Rat) 24684
steroid biosynthetic process
cell surface receptor signaling pathway
activation of transmembrane receptor protein tyrosine kinase activity
embryo implantation
lactation
regulation of cell adhesion
regulation of epithelial cell differentiation
prolactin signaling pathway
T cell activation
activation of JAK2 kinase activity
negative regulation of apoptotic process
JAK-STAT cascade involved in growth hormone signaling pathway
mammary gland epithelial cell differentiation
prostate gland growth
mammary gland alveolus development
JAK-STAT cascade
cytokine-mediated signaling pathway
mammary gland epithelium development
