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Protocadherin Gamma Antibody (MA5-45660) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Protocadherin Gamma (pan) monoclonal antibody (Product # MA5-45660) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1... View More
产品信息
MA5-45660
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
N159/5
抗原
Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Protocadherin-gamma-A1 that is shared by all 22 Gamma-protocadherins (A subfamily amino acids ~807-930, B subfamily amino acids ~789-912 and C subfamily amino acids ~818-941).
偶联物
PerCP
激发/发射光谱
482/675 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2932114
产品详细信息
1 µg/mL of MA5-45660 was sufficient for detection of Protocadherin gamma (pan) in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 100kDa. Cross-reacts with all Gamma-protocadherin-A, -B and -C proteins.
This antibody was formerly sold as clone S159-5.
靶标信息
The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 3' RACE product; cadherin 20; cadherin 21; cadherin ME3; cadherin ME6; Cadherin-20; Cadherin-21; cadherin-like 2; fibroblast cadherin FIB2; fibroblast cadherin FIB3; Fibroblast cadherin-2; Fibroblast cadherin-3; Pan-Gamma-Protocadherin-A; PC-43; PCDH gamma A; PCDH-gamma-A; PCDH-gamma-A1; PCDH-gamma-A10; PCDH-gamma-A11; PCDH-gamma-A12; PCDH-gamma-A2; PCDH-gamma-A3; PCDH-gamma-A4; PCDH-gamma-A5; PCDH-gamma-A6; PCDH-gamma-A7; PCDH-gamma-A8; PCDH-gamma-A9; PCDH-gamma-B1; PCDH-gamma-B2; PCDH-gamma-B3; PCDH-gamma-B4; PCDH-gamma-B5; PCDH-gamma-B6; PCDH-gamma-B7; PCDH-gamma-C3; PCDH-gamma-C4; PCDH-gamma-C5; PCDHGA; pcdhga4; protocadherin 13; protocadherin 2; protocadherin 43; protocadherin gamma a1; protocadherin gamma A12; protocadherin gamma a2; protocadherin gamma B2; protocadherin gamma B6; protocadherin gamma b7; protocadherin gamma C-VI; protocadherin gamma c5; protocadherin gamma subfamily A, 10; protocadherin gamma subfamily A, 12; protocadherin gamma subfamily A, 3; protocadherin gamma subfamily A, 4; protocadherin gamma subfamily B, 1, isoform 2; protocadherin gamma subfamily B, 5; protocadherin gamma subfamily B, 7; protocadherin gamma subfamily C, 3; Protocadherin gamma-A1; Protocadherin gamma-A10; Protocadherin gamma-A11; Protocadherin gamma-A12; Protocadherin gamma-A2; Protocadherin gamma-A3; Protocadherin gamma-A4; Protocadherin gamma-A5; Protocadherin gamma-A6; protocadherin gamma-A6-like; Protocadherin gamma-A7; Protocadherin gamma-A8; Protocadherin gamma-A9; Protocadherin gamma-B1; Protocadherin gamma-B2; Protocadherin gamma-B3; Protocadherin gamma-B4; protocadherin gamma-B4-like; Protocadherin gamma-B5; Protocadherin gamma-B6; protocadherin gamma-B6-like; Protocadherin gamma-B7; Protocadherin gamma-C3; Protocadherin gamma-C4; Protocadherin gamma-C5; Protocadherin-2; Protocadherin-43
基因别名: CDH-GAMMA-A5; CDH20; CDH21; FIB2; FIB3; KIAA0327; KIAA0588; ME3; ME6; mKIAA0588; mKIAA4054; pc2c; PC43; PCDH-GAMMA-A1; PCDH-GAMMA-A10; PCDH-GAMMA-A11; PCDH-GAMMA-A12; PCDH-GAMMA-A2; PCDH-GAMMA-A3; PCDH-GAMMA-A4; PCDH-GAMMA-A5; PCDH-GAMMA-A6; PCDH-GAMMA-A7; PCDH-GAMMA-A8; PCDH-GAMMA-A9; PCDH-GAMMA-B1; PCDH-GAMMA-B2; PCDH-GAMMA-B3; PCDH-GAMMA-B4; PCDH-GAMMA-B5; PCDH-GAMMA-B6; PCDH-GAMMA-B7; PCDH-GAMMA-C3; PCDH-GAMMA-C4; PCDH-GAMMA-C5; Pcdh13; PCDH2; PCDHGA1; PCDHGA10; Pcdhga10temp; PCDHGA11; PCDHGA12; PCDHGA2; PCDHGA3; PCDHGA4; PCDHGA5; PCDHGA6; PCDHGA7; PCDHGA8; PCDHGA9; PCDHGB1; PCDHGB2; PCDHGB3; PCDHGB4; PCDHGB5; PCDHGB6; PCDHGB7; PCDHGC3; PCDHGC4; PCDHGC5; UNQ371/PRO707
UniProt ID: (Human) Q9Y5H4, (Human) Q9Y5H3, (Human) Q9Y5H2, (Human) O60330, (Human) Q9Y5H1, (Human) Q9Y5H0, (Human) Q9Y5G9, (Human) Q9Y5G8, (Human) Q9Y5G7, (Human) Q9Y5G6, (Human) Q9Y5G5, (Human) Q9Y5G4, (Human) Q9Y5G3, (Human) Q9Y5G2, (Human) Q9Y5G1, (Human) O15099, (Human) Q9Y5G0, (Human) Q9Y5F9, (Human) Q9Y5F8, (Human) Q9UN70, (Human) Q9Y5F7, (Human) Q9Y5F6, (Mouse) Q91XY4
Entrez Gene ID: (Rat) 102552497, (Rat) 102552705, (Rat) 102552796, (Human) 56114, (Human) 56106, (Human) 56105, (Human) 26025, (Human) 56113, (Human) 56112, (Human) 56111, (Human) 56110, (Human) 56109, (Human) 56108, (Human) 9708, (Human) 56107, (Human) 56104, (Human) 56103, (Human) 56102, (Human) 8641, (Human) 56101, (Human) 56100, (Human) 56099, (Human) 5098, (Human) 56098, (Human) 56097, (Rat) 553129, (Mouse) 93709, (Rat) 498849, (Mouse) 93722, (Rat) 252897, (Mouse) 93723, (Mouse) 93724, (Rat) 498850, (Rat) 498846, (Mouse) 93710, (Mouse) 93711, (Rat) 498847, (Mouse) 93712, (Rat) 252894, (Rat) 291637, (Mouse) 93713, (Mouse) 93714, (Mouse) 93715, (Rat) 291635, (Rat) 364843, (Mouse) 93716, (Rat) 252895, (Mouse) 93717, (Mouse) 93699, (Mouse) 93700, (Mouse) 93701, (Mouse) 93702, (Rat) 680448, (Mouse) 93703, (Mouse) 93704, (Rat) 364844, (Mouse) 93706, (Rat) 116782, (Mouse) 93707, (Mouse) 93708, (Rat) 641525
