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Pyruvate Kinase Antibody (200-1178-0100) in WB
Western Blot of Goat Anti-Pyruvate Kinase Antibody. Lane 1: CHO K1 Lysate 10µg. Lane 2: CHO S Lysate 10µg. Lane 3: Opal Pre-stained Molecular Weight Marker (p/n MB-210-0500). Primary Antibody: Goat Anti-Pyruvate Kinase at 1:50 overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat IgG CY5 conjugate (p/n 605-710-125) at 1:10,000 for 30 mins at RT. Expect: ~57kDa.
产品信息
200-1178-0100
应用
建议稀释比
已发表文章
产品规格
种属反应
Rabbit
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Pyruvate Kinase (Rabbit Muscle)
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Ion-exchange chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
产品详细信息
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-PYRUVATE KINASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Pyruvate Kinase (Rabbit Muscle). This product has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross-reactivity against pyruvate kinase from other mammalian tissues is expected but has not been specifically determined.
靶标信息
PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. PKM2 has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms of PKM2 have been reported.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ATP:pyruvate 2-o-phosphotransferase; isozymes M1/M2; m2pk; Pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle isozyme; Pyruvate kinase PKM; pyruvate kinase, muscle; Threonine-protein kinase PKM2; tu; Tyrosine-protein kinase PKM2
基因别名: PKM; PKM2
UniProt ID: (Rabbit) P11974
Entrez Gene ID: (Rabbit) 100008676
