Invitrogen

RAD54L2 Polyclonal Antibody

RAD54L2 Antibody in Immunocytochemistry (ICC/IF)

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RAD54L2 Antibody (PA5-89311) in ICC/IF

Immunofluorescence analysis of RAD54L2 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with RAD54L2 Polyclonal Antibody (Product # PA5-89311) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # ... View More

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产品信息

PA5-89311

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

1:50-1:200

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 1178-1467 of human RAD54L2 (NP_0559212)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2805484

产品详细信息

Immunogen sequence: ELQQYADVAA ARESRQSSPS TNAALPGPPA QLMDSSAVPG TALGTEPRLG GHCLNSSLLV TGQPCGDRHP VLDLRGHKRK LATPPAAQES SRRRSRKGHL PAPVQPYEHG YPVSGGFAMP PVSLNHNLTT PFTSQAGENS LFMGSTPSYY QLSNLLADAR LVFPVTTDPL VPAGPVSSSS TATSVTASNP SFMLNPSVPG ILPSYSLPFS QPLLSEPRMF APFPSPVLPS NLSRGMSIYP GYMSPHAGYP AGGLLRSQVP PFDSHEVAEV GFSSNDDEDK DDDVIEVTGK

靶标信息

Adrenergic receptors (ARs) include four general types (a1, a2, b1 and b2) that are found in different target tissues and differ in their affinities and responses to various agonists and antagonists. The coupling of ARs to specific intracellular effectors is mediated through diverse heterotrimeric G proteins. ARs play a critical role in the development of prostate cancer, and transcriptional activity of AR is partly regulated by coregulatory proteins. RAD54L2 (RAD54-like 2), also known as ARIP4 (androgen receptor-interacting protein 4), HSPC325 or SRISNF2L, is a 1,467 amino acid nuclear protein belonging to the SNF2/RAD54 helicase family that consists of one helicase ATP-binding domain and a helicase C-terminal domain. RAD54L2 is a DNA helicase that regulates androgen receptor (AR)-dependent transactivation in a promoter-dependent manner. RAD54L2 is post-translationally sumoylated or phosphorylated upon DNA damage.

仅用于科研。不用于诊断过程。未经明确授权不得转售。