Invitrogen

RAP1A Monoclonal Antibody (5F8)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

查看其他9个RAP1A抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (4)
高级验证 (1)

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RAP1A Antibody (MA5-17164) in WB

Western blot was performed using Anti-RAP1A Monoclonal Antibody (5F8) (Product # MA5-17164) and 20 kDa band corresponding to RAP1A was observed across the cell lines tested except C2C12 in which the expression was observed upon myoblast differentiation. Whole cell lysates (30ug lysate) of C2C12 (Lane 1), C2C12 differentiated to Myoblasts (Lane2), HT-29 (Lane 3), MDA-MB-231 (Lane 4), MCF 10A (Lane 5), A-431 (Lane 6), HeLa (Lane 7) and A-375 (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolve... View More

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RAP1A Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. The expression was observed in all the cell lines except in undifferentiated C2C12 cells using RAP1A Monoclonal Antibody (5F8) (Product # MA5-17164) in Western Blot. {RE}

产品信息

MA5-17164

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

流式细胞分析 (Flow)

1:200-1:400

酶联免疫吸附实验 (ELISA)

1:10,000

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

5F8

抗原

Purified recombinant fragment of human RAP1A expressed in E. Coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2538635

产品详细信息

MA5-17164 targets RAP1A in FACS, indirect ELISA, and WB applications and shows reactivity with Human samples.

The MA5-17164 immunogen is purified recombinant fragment of human RAP1A expressed in E. Coli.

MA5-17164 detects RAP1A which has a predicted molecular weight of approximately 21kDa.

靶标信息

The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。