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Application
FIGURE: 1 / 4
RBM8A Antibody (PA5-78305) in ICC/IF
RBM8A Polyclonal Antibody detects RBM8A protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RBM8A protein stained by RBM8A Polyclonal Antibody (Product # PA5-78305) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-78305
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human RBM8A. The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.46 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2736513
Product Specific Information
Positive Control: 293T, A431, HeLa, HepG2
Predicted Reactivity: Mouse (100%), Zebrafish (93%), Japanese Medaka (85%), Xenopus laevis (93%), Bovine (98%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. Two alternative start codons result in two forms of the protein, and this gene also uses multiple polyadenylation sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Binder of OVCA1-1; BOV-1; BOV1; BOV1A; BOV1B; BOV1C; ribonucleoprotein RBM8; Ribonucleoprotein RBM8A; RNA binding motif protein 8; RNA binding motif protein 8B; rna-binding; RNA-binding motif protein 8A; RNA-binding protein 8A; RNA-binding protein Y14
Gene Aliases: BOV-1A; BOV-1B; BOV-1C; C1DELq21.1; DEL1q21.1; HSPC114; MDS014; RBM8; RBM8A; RBM8B; TAR; Y14; ZNRP; ZRNP1
UniProt ID: (Human) Q9Y5S9, (Rat) Q27W01
Entrez Gene ID: (Human) 9939, (Rat) 295284
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
regulation of alternative mRNA splicing, via spliceosome
mRNA splicing, via spliceosome
termination of RNA polymerase II transcription
RNA export from nucleus
mRNA export from nucleus
regulation of translation
mRNA 3'-end processing
mRNA processing
RNA splicing
mRNA transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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