Invitrogen

RBPJ Polyclonal Antibody

查看其他11个RBPJ抗体

抗体检测数据 (2)

RBPJ Antibody in Western Blot (WB)

RBPJ Antibody in Western Blot (WB)

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RBPJ Antibody (PA5-22226) in WB

Western Blot analysis of RBPJ was performed by separating 30 µg of Jurkat lysates by 7.5% SDS PAGE. Proteins were transferred to a membrane and probed with a RBPJ Polyclonal Antibody (Product # PA5-22226) at a dilution of 1:2000.

RBPJ Antibody in Western Blot (WB)

RBPJ Antibody in Western Blot (WB)

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RBPJ Antibody in Western Blot (WB) — Western Blot analysis of RBPJ was performed by separating 30 µg of Jurkat lysates by 7.5% SDS PAGE. Proteins were transferred to a membrane and probed with a RBPJ Polyclonal Antibody (Product # PA5-22226) at a dilution of 1:2000.

RBPJ Antibody in Western Blot (WB) — Western Blot using RBPJ Polyclonal Antibody (Product # PA5-22226). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RBPJ Polyclonal Antibody (Product # PA5-22226) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody,.

产品信息

PA5-22226

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:3,000

-

免疫细胞化学 (ICC/IF)

1:100-1:1,000

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 258 and 500 of RBP-Jkappa (Uniprot ID#Q06330)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.75 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_11155040

产品详细信息

Recommended positive controls: 293T, A431, HeLa, Molt-4, Raji.

Predicted reactivity: Mouse (97%), Rat (98%), Xenopus laevis (93%), Rhesus Monkey (99%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

CSL plays a role in the regulatory network through which muscle cells coordinate their structural and functional states during growth, adaptation, and repair. The protein is abundantly expressed in heart and skeletal muscle and expression is increased by passive stretch. Mutations in this gene can lead to X-linked deafness.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CBF-1; H-2K binding factor-2; immunoglobulin kappa J region recombination signal binding protein 1; J kappa-recombination signal-binding protein; RBP-J; RBP-J kappa; RBP-JK; rbp-jkappa; Recombining binding protein suppressor of hairless; Renal carcinoma antigen NY-REN-30; suppressor of hairless homolog

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基因别名: AI843960; AOS3; CBF1; csl; IGKJRB; IGKJRB1; Igkrsbp; KBF2; RBP-J; RBP-J kappa; RBPJ; RBPJK; RBPSUH; SUH

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UniProt ID: (Human) Q06330, (Mouse) P31266

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Entrez Gene ID: (Human) 3516, (Mouse) 19664

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recombinase activity RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II repressing transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding sequence-specific DNA binding protein N-terminus binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding protein complex binding DNA-binding transcription factor

参与通路

negative regulation of transcription from RNA polymerase II promoter angiogenesis somitogenesis epithelial to mesenchymal transition blood vessel remodeling inflammatory response to antigenic stimulus secondary heart field specification outflow tract morphogenesis endocardium morphogenesis epithelial to mesenchymal transition involved in endocardial cushion formation cardiac left ventricle morphogenesis ventricular trabecula myocardium morphogenesis regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation DNA recombination transcription initiation from RNA polymerase II promoter humoral immune response Notch signaling pathway positive regulation of transcription of Notch receptor target negative regulation of cell proliferation auditory receptor cell fate commitment epidermal cell fate specification pituitary gland development B cell differentiation keratinocyte differentiation negative regulation of ossification positive regulation of BMP signaling pathway somatic stem cell population maintenance dorsal aorta morphogenesis atrioventricular canal development defense response to bacterium myeloid dendritic cell differentiation negative regulation of cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of timing of cell differentiation sebaceous gland development hair follicle maturation positive regulation of cardiac muscle cell proliferation Clara cell differentiation labyrinthine layer blood vessel development arterial endothelial cell fate commitment Notch signaling involved in heart development positive regulation of transcription from RNA polymerase II promoter in response to hypoxia blood vessel lumenization interleukin-4 secretion blood vessel endothelial cell fate specification positive regulation of ERBB signaling pathway positive regulation of ephrin receptor signaling pathway positive regulation of canonical Wnt signaling pathway involved in cardiac muscle cell fate commitment positive regulation of cell proliferation involved in heart morphogenesis heart looping endocardium development transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter heart development positive regulation of cell proliferation regulation of gene expression positive regulation of gene expression negative regulation of neuron projection development negative regulation of smooth muscle cell migration hemopoiesis neuron differentiation negative regulation of smooth muscle cell apoptotic process positive regulation of Notch signaling pathway involved in heart induction cell fate commitment positive regulation of transcription, DNA-templated positive regulation of smooth muscle cell proliferation artery morphogenesis Notch signaling pathway involved in arterial endothelial cell fate commitment regulation of cell adhesion involved in heart morphogenesis determination of heart left/right asymmetry