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RELM beta Antibody (500-P215-1MG) in WB
Western Blot: To detect Murine RELMbeta by Western Blot analysis RELM beta Polyclonal Antibody (Product # 500-P215-1MG) can be used at a concentration of 0.1-0.2 µg/mL. when used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine RELMbeta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P215-1MG
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Murine RELMbeta
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2929856
产品详细信息
AA Sequence of recombinant protein: MQCSFESLVD QRIKEALSRQ EPKTISCTSV TSSGRLASCP AGMVVTGCAC GYGCGSWDIR NGNTCHCQCS VMDWASARCC RMA.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine RELMbeta. Anti-Murine RELMbeta-specific antibody was purified by affinity chromatography employing an immobilized Murine RELMbeta matrix.
Sandwich ELISA: To detect Murine RELMbeta by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine RELMbeta (500-P215Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine RELMbeta.
Western Blot: To detect Murine RELMbeta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. when used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine RELMbeta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
Adipose tissue is an energy reserve that is strictly regulated in animals. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), perilipin, and resistin. Resistin, also known as Adipocyte Secreted Factor (ADSF) and FIZZ3, is a cytokine that is specifically secreted by adipocytes. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes. Studies show that in obese, diabetic mice the levels of serum resistin are increased, which has an antagonistic effect on insulin. Additional studies have shown that resistin causes insulin resistance and glucose intolerance in mice. Resistin gene expression requires C/EBP alpha, which is necessary and sufficient for expression. Studies show that the coactivators CBP and p300, which have been shown to display histone acetyltransferase (HAT) activity, abundantly acetylate the histones at the resistin promoter suggesting their role in activating chromatin for resistin transcription. Numerous PPAR gamma and RXR ligands have been shown to downregulate resistin expression in adipocytes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Cysteine-rich secreted protein A12-beta; Cysteine-rich secreted protein FIZZ2; found in inflammatory zone 2; RELMbeta; Resistin-like beta; resistin-like molecule beta; RSNB
基因别名: 9030012B21Rik; Fizz2; Relmb; RELMbeta; Retnlb; Xcp3
UniProt ID: (Mouse) Q99P86
Entrez Gene ID: (Mouse) 57263
