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RFC1 Antibody (A300-320A) in WB
Detection of human RFC1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RFC1 antibody A300-320A (lot A300-320A-2) used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. RFC1 was also immunoprecipitated by a prior lot (lot A300-320A-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B)... View More
产品信息
A300-320A
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Region between residue 1100 and the C-terminus (residue 1148) of Human Replication Factor C subunit 1.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
phosphate/tris citrate, pH 7-8
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Wet ice
产品详细信息
The recommended shelf life for this product is 1 year from date of receipt.
靶标信息
Replication factor C (RFC) is an essential DNA polymerase accessory protein that is required for numerous aspects of DNA metabolism, including DNA replication, DNA repair and telomere metabolism. RFC is a heteropentameric complex that recognizes a primer on a template DNA, binds to a primer terminus and loads proliferating cell nuclear antigen (PCNA) onto DNA at primer-template junctions in an ATP-dependent reaction. All five of the RFC subunits share a set of related sequences (RFC boxes) that include nucleotide binding consensus sequences. Four of the five RFC genes (RFC1, RFC2, RFC3 and RFC4) have consensus ATP-binding motifs. The small RFC proteins, RFC2, RFC3, RFC4 and RFC5, interact with Rad24, whereas the RFC1 subunit does not. RFC2, the third-largest subunit of the RFC complex, exhibits ATP binding which makes it important for both DNA replication and checkpoint function.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: A1 140 kDa subunit; Activator 1 140 kDa subunit; Activator 1 large subunit; Activator 1 subunit 1; DNA-binding protein PO-GA; MHC binding factor, beta; replication factor C (activator 1) 1, 145kDa; Replication factor C 140 kDa subunit; Replication factor C large subunit; Replication factor C subunit 1; replication factor C1; RF-C 140 kDa subunit
基因别名: A1; MHCBFB; PO-GA; RECC1; RFC; RFC1; RFC140
UniProt ID: (Human) P35251
Entrez Gene ID: (Human) 5981
telomere maintenance via recombination
DNA-dependent DNA replication
transcription-coupled nucleotide-excision repair
nucleotide-excision repair, DNA incision, 5'-to lesion
nucleotide-excision repair, DNA gap filling
transcription, DNA-templated
regulation of transcription, DNA-templated
telomere maintenance via telomerase
translesion synthesis
nucleotide-excision repair, DNA incision
error-prone translesion synthesis
DNA damage response, detection of DNA damage
positive regulation of catalytic activity
error-free translesion synthesis
